The Measurement of Swimming Velocity of Vibrio cholerae and Pseudomonas aeruginosa Using the Video Tracking Method

The swimming velocities of two monotrichous flagellated bacteria were measured by a computer-assisted video tracking method. Tracing the moving path of the individual bacterium revealed that the bacterial cell did not swim continuously in a straight direction, but frequently changed swimming directi...

Full description

Saved in:
Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1995, Vol.39(10), pp.741-744
Main Authors: Shigematsu, Mika, Meno, Yuko, Misumi, Hiroyasu, Amako, Kazunobu
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microbiology
Motility
Motility, taxis
Pseudomonas aeruginosa
Swimming velocity
Vibrio cholerae
Video tracking
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The swimming velocities of two monotrichous flagellated bacteria were measured by a computer-assisted video tracking method. Tracing the moving path of the individual bacterium revealed that the bacterial cell did not swim continuously in a straight direction, but frequently changed swimming direction and velocity. The average swimming velocities calculated from the 3-sec path were 75.4±9.4μm/sec in four strains of Vibrio cholerae and 51.3±8.4μm/sec in five strains of Pseudomonas aeruginosa. These results suggest that V. cholerae swim faster than P. aeruginosa at 30C in nutrient broth. This method is useful for a detailed analysis of bacterial movement and moving patterns in different environmental conditions.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1995.tb03260.x