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The Measurement of Swimming Velocity of Vibrio cholerae and Pseudomonas aeruginosa Using the Video Tracking Method

The swimming velocities of two monotrichous flagellated bacteria were measured by a computer-assisted video tracking method. Tracing the moving path of the individual bacterium revealed that the bacterial cell did not swim continuously in a straight direction, but frequently changed swimming directi...

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Published in:MICROBIOLOGY and IMMUNOLOGY 1995, Vol.39(10), pp.741-744
Main Authors: Shigematsu, Mika, Meno, Yuko, Misumi, Hiroyasu, Amako, Kazunobu
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Language:English
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description The swimming velocities of two monotrichous flagellated bacteria were measured by a computer-assisted video tracking method. Tracing the moving path of the individual bacterium revealed that the bacterial cell did not swim continuously in a straight direction, but frequently changed swimming direction and velocity. The average swimming velocities calculated from the 3-sec path were 75.4±9.4μm/sec in four strains of Vibrio cholerae and 51.3±8.4μm/sec in five strains of Pseudomonas aeruginosa. These results suggest that V. cholerae swim faster than P. aeruginosa at 30C in nutrient broth. This method is useful for a detailed analysis of bacterial movement and moving patterns in different environmental conditions.
doi_str_mv 10.1111/j.1348-0421.1995.tb03260.x
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ispartof MICROBIOLOGY and IMMUNOLOGY, 1995, Vol.39(10), pp.741-744
issn 0385-5600
1348-0421
language eng
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source Alma/SFX Local Collection
subjects Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microbiology
Motility
Motility, taxis
Pseudomonas aeruginosa
Swimming velocity
Vibrio cholerae
Video tracking
title The Measurement of Swimming Velocity of Vibrio cholerae and Pseudomonas aeruginosa Using the Video Tracking Method
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