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The Measurement of Swimming Velocity of Vibrio cholerae and Pseudomonas aeruginosa Using the Video Tracking Method
The swimming velocities of two monotrichous flagellated bacteria were measured by a computer-assisted video tracking method. Tracing the moving path of the individual bacterium revealed that the bacterial cell did not swim continuously in a straight direction, but frequently changed swimming directi...
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Published in: | MICROBIOLOGY and IMMUNOLOGY 1995, Vol.39(10), pp.741-744 |
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cites | cdi_FETCH-LOGICAL-c7040-a19b82eb6fdb451a1c1c1805ed8a670e17fd796a3337899a2058063d18397a913 |
container_end_page | 744 |
container_issue | 10 |
container_start_page | 741 |
container_title | MICROBIOLOGY and IMMUNOLOGY |
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creator | Shigematsu, Mika Meno, Yuko Misumi, Hiroyasu Amako, Kazunobu |
description | The swimming velocities of two monotrichous flagellated bacteria were measured by a computer-assisted video tracking method. Tracing the moving path of the individual bacterium revealed that the bacterial cell did not swim continuously in a straight direction, but frequently changed swimming direction and velocity. The average swimming velocities calculated from the 3-sec path were 75.4±9.4μm/sec in four strains of Vibrio cholerae and 51.3±8.4μm/sec in five strains of Pseudomonas aeruginosa. These results suggest that V. cholerae swim faster than P. aeruginosa at 30C in nutrient broth. This method is useful for a detailed analysis of bacterial movement and moving patterns in different environmental conditions. |
doi_str_mv | 10.1111/j.1348-0421.1995.tb03260.x |
format | article |
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Tracing the moving path of the individual bacterium revealed that the bacterial cell did not swim continuously in a straight direction, but frequently changed swimming direction and velocity. The average swimming velocities calculated from the 3-sec path were 75.4±9.4μm/sec in four strains of Vibrio cholerae and 51.3±8.4μm/sec in five strains of Pseudomonas aeruginosa. These results suggest that V. cholerae swim faster than P. aeruginosa at 30C in nutrient broth. This method is useful for a detailed analysis of bacterial movement and moving patterns in different environmental conditions.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.1995.tb03260.x</identifier><identifier>CODEN: MIIMDV</identifier><language>eng</language><publisher>Tokyo: Blackwell Publishing Ltd</publisher><subject>Bacteriology ; Biological and medical sciences ; Fundamental and applied biological sciences. 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Psychology</topic><topic>Microbiology</topic><topic>Motility</topic><topic>Motility, taxis</topic><topic>Pseudomonas aeruginosa</topic><topic>Swimming velocity</topic><topic>Vibrio cholerae</topic><topic>Video tracking</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shigematsu, Mika</creatorcontrib><creatorcontrib>Meno, Yuko</creatorcontrib><creatorcontrib>Misumi, Hiroyasu</creatorcontrib><creatorcontrib>Amako, Kazunobu</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shigematsu, Mika</au><au>Meno, Yuko</au><au>Misumi, Hiroyasu</au><au>Amako, Kazunobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Measurement of Swimming Velocity of Vibrio cholerae and Pseudomonas aeruginosa Using the Video Tracking Method</atitle><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle><addtitle>Microbiology and Immunology</addtitle><date>1995-10</date><risdate>1995</risdate><volume>39</volume><issue>10</issue><spage>741</spage><epage>744</epage><pages>741-744</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><coden>MIIMDV</coden><abstract>The swimming velocities of two monotrichous flagellated bacteria were measured by a computer-assisted video tracking method. Tracing the moving path of the individual bacterium revealed that the bacterial cell did not swim continuously in a straight direction, but frequently changed swimming direction and velocity. The average swimming velocities calculated from the 3-sec path were 75.4±9.4μm/sec in four strains of Vibrio cholerae and 51.3±8.4μm/sec in five strains of Pseudomonas aeruginosa. These results suggest that V. cholerae swim faster than P. aeruginosa at 30C in nutrient broth. This method is useful for a detailed analysis of bacterial movement and moving patterns in different environmental conditions.</abstract><cop>Tokyo</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1348-0421.1995.tb03260.x</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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language | eng |
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source | Alma/SFX Local Collection |
subjects | Bacteriology Biological and medical sciences Fundamental and applied biological sciences. Psychology Microbiology Motility Motility, taxis Pseudomonas aeruginosa Swimming velocity Vibrio cholerae Video tracking |
title | The Measurement of Swimming Velocity of Vibrio cholerae and Pseudomonas aeruginosa Using the Video Tracking Method |
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