Turn-ON fluorescent affinity labeling using a small bifunctional O-nitrobenzoxadiazole unit

Affinity labeling has become a powerful tool to identify target proteins, as well as to visualize/characterize target functions in living cells. However, although various functional groups have been utilized for affinity labeling, many of them have disadvantages such as complex structure and large s...

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Bibliographic Details
Published in:Chemical science (Cambridge) 2014, Vol.5 (3), p.1021-1029
Main Authors: Yamaguchi, Takao, Asanuma, Miwako, Nakanishi, Shuichi, Saito, Yohei, Okazaki, Masateru, Dodo, Kosuke, Sodeoka, Mikiko
Format: Article
Language:English
Subjects:
Affinity
Attachment
Channels
Ligands
Marking
Mitochondria
Proteins
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Summary:Affinity labeling has become a powerful tool to identify target proteins, as well as to visualize/characterize target functions in living cells. However, although various functional groups have been utilized for affinity labeling, many of them have disadvantages such as complex structure and large size. To address this problem, we designed a simple chemical probe bearing a small bifunctional O-NBD unit (NBD: nitrobenzoxadiazole). Model ligand-protein experiments showed that the O-NBD unit has excellent characteristics for target-specific labeling even in the presence of a large excess of non-target proteins. Moreover, attachment of the O-NBD unit to N,N-dialkyl-2-phenylindol-3-ylgly oxylamides (PIGAs), which are recently developed translocator protein (TSPO) ligands, enabled us to visualize mitochondria expressing TSPO in living cells by means of "turn-ON" fluorescence. Two-dimensional PAGE analysis of the labeled mouse kidney mitochondria strongly suggested that our synthetic probe selectively modified a partner protein of TSPO, voltage-dependent anion channel (VDAC).
ISSN:2041-6520
2041-6539
DOI:10.1039/c3sc52704b