Real-Time Monitoring of Auxin Vesicular Exocytotic Efflux from Single Plant Protoplasts by Amperometry at Microelectrodes Decorated with Nanowires

Recent biochemical results suggest that auxin (IAA) efflux is mediated by a vesicular cycling mechanism, but no direct detection of vesicular IAA release from single plant cells in real‐time has been possible up to now. A TiC@C/Pt‐QANFA micro‐electrochemical sensor has been developed with high sensi...

Full description

Saved in:
Bibliographic Details
Published in:Angewandte Chemie International Edition 2014-03, Vol.53 (10), p.2643-2647
Main Authors: Liu, Jun-Tao, Hu, Liang-Sheng, Liu, Yan-Ling, Chen, Rong-Sheng, Cheng, Zhi, Chen, Shi-Jing, Amatore, Christian, Huang, Wei-Hua, Huo, Kai-Fu
Format: Article
Language:English
Subjects:
amperometric monitoring
auxin efflux
Biochemistry
Carbon - chemistry
Cycles
Efflux
electrochemical sensors
Electrochemical Techniques
Exocytosis
Indoleacetic Acids - analysis
Microelectrodes
Monitoring
Nanowires
Nanowires - chemistry
Particle Size
Plants (organisms)
Plants - chemistry
Platinum - chemistry
Protons
Protoplasts
Real time
Sensors
single plant cells
Surface Properties
Time Factors
Titanium - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recent biochemical results suggest that auxin (IAA) efflux is mediated by a vesicular cycling mechanism, but no direct detection of vesicular IAA release from single plant cells in real‐time has been possible up to now. A TiC@C/Pt‐QANFA micro‐electrochemical sensor has been developed with high sensitivity in detection of IAA, and it allows real‐time monitoring and quantification of the quantal release of auxin from single plant protoplast by exocytosis. Auxin(IAA) efflux has been shown by recent biochemical results to be mediated by a vesicular cycling mechanism, but up to now there was no means for direct detection of IAA release from single plant cells in real‐time. A novel TiC@C/Pt‐QANFA micro‐electrochemical sensor has high sensitivity in detection of IAA, and allows real‐time monitoring and quantification of the quantal release of auxin from single plant protoplast by exocytosis.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201308972