Expanding the Genetic Code for Photoclick Chemistry in E.coli, Mammalian Cells, and A.thaliana

Breaking the (genetic) code: N- epsilon -acryl- lysine was genetically incorporated into proteins in bacterial cells, mammalian cells, and Arabidopsis thaliana by co-expressing a pyrrolysyl-tRNA mutant synthetase and its cognate tRNA in the model organism. A photoclick reaction driven by irradiation...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2013-09, Vol.52 (37), p.9700-9704
Main Authors: Li, Fahui, Zhang, Hua, Sun, Yun, Pan, Yanchao, Zhou, Juanzuo, Wang, Jiangyun
Format: Article
Language:English
Subjects:
Arabidopsis thaliana
Bacteria
Genetic code
Genetics
Lasers
Light (visible radiation)
Lysine
Organisms
Proteins
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Summary:Breaking the (genetic) code: N- epsilon -acryl- lysine was genetically incorporated into proteins in bacterial cells, mammalian cells, and Arabidopsis thaliana by co-expressing a pyrrolysyl-tRNA mutant synthetase and its cognate tRNA in the model organism. A photoclick reaction driven by irradiation by 365nm UV light or 405nm laser light and allowed for site-specific protein labeling within minutes both invitro and invivo.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201303477