Promotion of neural sprouting using low-level green light-emitting diode phototherapy

We irradiated neuroblastoma SH-SY5Y cell line with low-level light-emitting diode (LED) illumination at a visible wavelength of 520 nm (green) and intensity of 100  mW/cm2. We captured and analyzed the cell morphology before LED treatment, immediately after, and 12 and 24 h after treatment. Our stud...

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Bibliographic Details
Published in:Journal of biomedical optics 2015-02, Vol.20 (2), p.020502-020502
Main Authors: Alon, Noa, Duadi, Hamootal, Cohen, Ortal, Samet, Tamar, Zilony, Neta, Schori, Hadas, Shefi, Orit, Zalevsky, Zeev
Format: Article
Language:English
Subjects:
Animals
Biotechnology
Cell Line, Tumor
Elongation
Gene expression
Genes
Illumination
Light
Light-emitting diodes
Lighting
Models, Biological
Neural networks
Neurites - radiation effects
Neurons - radiation effects
Phototherapy
Promotion
Wavelengths
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Summary:We irradiated neuroblastoma SH-SY5Y cell line with low-level light-emitting diode (LED) illumination at a visible wavelength of 520 nm (green) and intensity of 100  mW/cm2. We captured and analyzed the cell morphology before LED treatment, immediately after, and 12 and 24 h after treatment. Our study demonstrated that LED illumination increases the amount of sprouting dendrites in comparison to the control untreated cells. This treatment also resulted in more elongated cells after treatment in comparison to the control cells and higher levels of expression of a differentiation related gene. This result is a good indication that the proposed method could serve in phototherapy treatment for increasing sprouting and enhancing neural network formation.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.20.2.020502