Achieving room temperature DNA amplification by dialling in destabilization

The ability to amplify nucleic acid biomarkers at room temperature has remained elusive despite the great need of diagnostics suitable for the point of care. To exponentially amplify DNA within a wide range of ambient temperatures (18-26 °C), we explore combining two destabilizing elements in our is...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2015-06, Vol.51 (44), p.9101-9104
Main Authors: Alladin-Mustan, B Safeenaz, Mitran, Catherine J, Gibbs-Davis, Julianne M
Format: Article
Language:English
Subjects:
Ambient temperature
Amplification
Base Pair Mismatch
Benches
Biomarkers
Deoxyribonucleic acid
Destabilization
Diagnostic systems
DNA - genetics
Heat sources
Nucleic Acid Amplification Techniques
Nucleic acids
Point-of-Care Systems
Temperature
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Summary:The ability to amplify nucleic acid biomarkers at room temperature has remained elusive despite the great need of diagnostics suitable for the point of care. To exponentially amplify DNA within a wide range of ambient temperatures (18-26 °C), we explore combining two destabilizing elements in our isothermal lesion-induced DNA amplification system. We demonstrate rapid DNA amplification at the bench without a heat source.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc01548k