STUDIES ON THE METABOLIC PATHWAY OF ACONITINE IN RABBIT AND HUMAN USING ELECTROSPRAY IONIZATION-MASS SPECTROMETRY

Aconitine is the main active component of the genus of aconitum plants, which are widely used in clinics in China. To clarify the toxicity and bioactivity of aconitine, the metabolic pathway is an important and key point. After oral administration of aconitine (5 mg · kg −1 ) to male rabbits, the li...

Full description

Saved in:
Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2013-04, Vol.36 (12), p.1686-1696
Main Authors: Liu, Yong-Gang, Sun, Ming-Qian, Zhang, Hong-Gui
Format: Article
Language:English
Subjects:
aconitine
Aconitum
Biocompatibility
electrospray ionization
HPLC
Human
in vivo
Ionization
Liquid chromatography
mass spectrometry
metabolic pathway
Metabolites
Pathways
Rabbits
Toxicity
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aconitine is the main active component of the genus of aconitum plants, which are widely used in clinics in China. To clarify the toxicity and bioactivity of aconitine, the metabolic pathway is an important and key point. After oral administration of aconitine (5 mg · kg −1 ) to male rabbits, the livers were pretreated by using a solid-phase extraction technique (SPE) with C 18 cartridges, and a LC/MS/MS method with electrospray ionization (ESI), positive ion mode, and collision induced dissociation (CID) was used to elucidate the structures of the major metabolites of aconitine. One new metabolite was found; the metabolic pathway was calculated. This result was reproduced in humans who took a medical liquor containing Chuanwu (Aconitum carmichaeli Debx.). In this article, we found two new metabolites; their protonated molecular ions were at m/z 468 and m/z 482, and multistage fragment ions with neutral loss of 18 u (H 2 O) and 32 u (CH 3 OH). They were detected in vivo, and were labeled dehydra-aconine and dehydra-hypaconine, respectively. The possible metabolic pathway was proposed for the first time.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826076.2012.695315