Rapidly Reversible Manipulation of Molecular Activity with Dual Chemical Dimerizers

Tell it where to go: Rapamycin induced the relocation of an FRB‐fused protein of interest (POI) to the plasma membrane (labeled with the fusion protein GAIs–FKBP–C2(LACT)) to activate a signaling event (see picture). Subsequent treatment with a gibberellic acid ester led to the relocation of the who...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2013-06, Vol.52 (25), p.6450-6454
Main Authors: Lin, Yu-Chun, Nihongaki, Yuta, Liu, Tzu-Yu, Razavi, Shiva, Sato, Moritoshi, Inoue, Takanari
Format: Article
Language:English
Subjects:
cell signaling
chemically inducible dimerization
Dimerization
enzymes
Esters
Gibberellic acid
HEK293 Cells
Humans
Membranes
Mitochondria
phosopholipids
Pictures
Proteins
Rapamycin
Relocation
reversible perturbation
Signal Transduction
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Summary:Tell it where to go: Rapamycin induced the relocation of an FRB‐fused protein of interest (POI) to the plasma membrane (labeled with the fusion protein GAIs–FKBP–C2(LACT)) to activate a signaling event (see picture). Subsequent treatment with a gibberellic acid ester led to the relocation of the whole GAIs–FKBP–C2(LACT)/rapamycin/FRB–POI complex to the Tom20–GID1‐labeled mitochondria with the termination of POI‐dependent signaling.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201301219