Top-Down Chemoenzymatic Approach to a High-Mannose-Type Glycan Library: Synthesis of a Common Precursor and Its Enzymatic Trimming

From the stacks: A novel method for construction of a high‐mannose‐type glycan library by systematic enzymatic trimming of a single synthetic Man9‐based precursor was developed. Efficient chemical synthesis of the tetradecasaccharide common precursor and orthogonal enzymatic trimming to obtain all M...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2013-07, Vol.52 (29), p.7426-7431
Main Authors: Koizumi, Akihiko, Matsuo, Ichiro, Takatani, Maki, Seko, Akira, Hachisu, Masakazu, Takeda, Yoichi, Ito, Yukishige
Format: Article
Language:English
Subjects:
Boron Compounds - chemistry
Carbohydrate Sequence
carbohydrates
Construction
Derivatives
enzymes
Glycan
glycopeptides
Glycoproteins - chemistry
Glycoproteins - metabolism
Glycoside Hydrolases - metabolism
Libraries
Mannose - chemistry
Molecular Sequence Data
Polysaccharides - chemical synthesis
Polysaccharides - chemistry
Precursors
Protein Folding
Stacks
Synthesis
synthesis design
Trimming
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Summary:From the stacks: A novel method for construction of a high‐mannose‐type glycan library by systematic enzymatic trimming of a single synthetic Man9‐based precursor was developed. Efficient chemical synthesis of the tetradecasaccharide common precursor and orthogonal enzymatic trimming to obtain all M8‐9 and G1M8‐9 derivatives was demonstrated. G=glucose, M=mannose.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201301613