Ligand- and pH-Induced Conformational Changes of RNA Domain Helix69 Revealed by 2-Aminopurine Fluorescence

Loop conformation: The loop of the RNA domain helix69 (H69) was modified with the fluorescent analogue 2-aminopurine (2AP), thus showing different conformational states under various conditions. The application of this model RNA reveals the unique impact of aminoglycoside neomycin, which differs fro...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2012-11, Vol.51 (48), p.12095-12098
Main Authors: Sakakibara, Yogo, Abeysirigunawardena, Sanjaya C, Duc, Anne-Cecile E, Dremann, Danielle N, Chow, Christine S
Format: Article
Language:English
Subjects:
Aminoglycosides
Analogue
Fluorescence
Mathematical models
Pictures
Ribonucleic acids
Online Access:Get full text
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Summary:Loop conformation: The loop of the RNA domain helix69 (H69) was modified with the fluorescent analogue 2-aminopurine (2AP), thus showing different conformational states under various conditions. The application of this model RNA reveals the unique impact of aminoglycoside neomycin, which differs from the effects of structurally related compounds paromomycin and gentamicin, on the H69 loop conformation in solution (see picture).
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201206000