Vinylsulfonamide and Acrylamide Modification of DNA for Cross-linking with Proteins

Bioorthogonal covalent cross‐linking of DNA‐binding proteins (p53) to DNA was achieved through novel DNA probes bearing a reactive vinylsulfonamide (VS) group. The VS‐modified dCTP served as building block for polymerase synthesis of modified DNA, which was readily conjugated with cysteine‐containin...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2013-09, Vol.52 (40), p.10515-10518
Main Authors: Dadová, Jitka, Orság, Petr, Pohl, Radek, Brázdová, Marie, Fojta, Miroslav, Hocek, Michal
Format: Article
Language:English
Subjects:
Acrylamide
Acrylamide - chemical synthesis
Acrylamide - chemistry
Bearing
bioorthogonal chemistry
Covalence
Cross-Linking Reagents - chemistry
Crosslinking
Deoxyribonucleic acid
DNA
DNA - chemical synthesis
DNA - chemistry
DNA polymerase
DNA-Directed DNA Polymerase - chemistry
Ethylenes - chemistry
Michael additions
Models, Molecular
Polymerase
Proteins
Proteins - chemistry
Proteins - metabolism
Sulfonamides - chemical synthesis
Sulfonamides - chemistry
Sulfonic Acids - chemistry
Synthesis
Vinyl Compounds - chemical synthesis
Vinyl Compounds - chemistry
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Summary:Bioorthogonal covalent cross‐linking of DNA‐binding proteins (p53) to DNA was achieved through novel DNA probes bearing a reactive vinylsulfonamide (VS) group. The VS‐modified dCTP served as building block for polymerase synthesis of modified DNA, which was readily conjugated with cysteine‐containing peptides and proteins by Michael addition.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201303577