Tomographic diffractive microscopy of living cells based on a common-path configuration

We demonstrate a common-path tomographic diffractive microscopy technique for three-dimensional (3D) refractive-index (RI) imaging of unstained living cells. A diffraction grating is utilized to generate a reference beam that traverses a blank region of the sample in a common-path off-axis interfero...

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Bibliographic Details
Published in:Optics letters 2014-04, Vol.39 (7), p.2210-2213
Main Authors: Hsu, Wei-Chen, Su, Jing-Wei, Tseng, Te-Yu, Sung, Kung-Bin
Format: Article
Language:English
Subjects:
Beams (radiation)
Blanks
Cell Survival
Diffraction
Diffraction gratings
Fluctuation
HeLa Cells
Humans
Illumination
Imaging, Three-Dimensional
Microscopy
Microscopy - methods
Polystyrenes - chemistry
Refractometry
Temporal logic
Three dimensional
Tomography - methods
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Summary:We demonstrate a common-path tomographic diffractive microscopy technique for three-dimensional (3D) refractive-index (RI) imaging of unstained living cells. A diffraction grating is utilized to generate a reference beam that traverses a blank region of the sample in a common-path off-axis interferometry setup. Single-shot phase images captured at multiple illumination angles are used for 3D RI reconstruction based on optical diffraction tomography. The common-path configuration shows lower temporal phase fluctuations and better RI resolution than a Mach-Zehnder configuration. 3D subcellular RI distributions of live HeLa cells are quantified.
ISSN:0146-9592
1539-4794
DOI:10.1364/ol.39.002210