A Microfluidic Technique for Quantification of Steroids in Core Needle Biopsies

Core needle biopsy (CNB) sampling is known to be inexpensive and minimally invasive relative to traditional tissue resectioning. But CNBs are often not used in analytical settings because of the tiny amount of sample and analyte. To address this challenge, we introduce an analytical method capable o...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2015-05, Vol.87 (9), p.4688-4695
Main Authors: Kim, Jihye, Abdulwahab, Sara, Choi, Kihwan, Lafrenière, Nelson M, Mudrik, Jared M, Gomaa, Hala, Ahmado, Hend, Behan, Lucy-Ann, Casper, Robert F, Wheeler, Aaron R
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Biopsy
Biopsy, Large-Core Needle
Breast
Chromatography
Chromatography, High Pressure Liquid
Hormone replacement therapy
Liquid chromatography
Mass spectrometry
Mathematical analysis
Microfluidic Analytical Techniques
Microfluidics
Multiplexing
Needles
Rats
Sampling
Steroids
Steroids - analysis
Tandem Mass Spectrometry
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Summary:Core needle biopsy (CNB) sampling is known to be inexpensive and minimally invasive relative to traditional tissue resectioning. But CNBs are often not used in analytical settings because of the tiny amount of sample and analyte. To address this challenge, we introduce an analytical method capable of multiplexed steroid quantification in CNB samplesthose studied here ranged in mass from 2 to 8 mg. The new method uses digital microfluidics to extract steroids from CNB tissue samples (including a solid-phase extraction cleanup step) followed by analysis by high-performance liquid chromatography tandem mass spectrometry (HPLC–MS/MS). The method has limits of detection of 3.6, 1.6, 5.8, and 8.5 fmol for estradiol, androstendione, testoterone, and progesterone, respectively. We propose that future generations of this method may be useful for regular quantification of steroids in core needle biopsy samples of breast tissue to inform dosage and timing of antihormone or hormone replacement therapies as part of a personalized medicine approach to treating a variety of hormone-sensitive disorders.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac5043297