Visualization of Oxygen Consumption of Single Living Cells by Scanning Electrochemical Microscopy: The Influence of the Faradaic Tip Reaction

The influence of the reaction rate at the SECM tip on the overall imaging result is often neglected during respiration studies performed by SECM. The effect of the driving force of the tip reaction is elucidated using a potential pulse profile implemented into a constant‐distance mode. Time‐dependen...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2013-06, Vol.52 (24), p.6335-6338
Main Authors: Nebel, Michaela, Grützke, Stefanie, Diab, Nizam, Schulte, Albert, Schuhmann, Wolfgang
Format: Article
Language:English
Subjects:
cell respiration
cells
Diffusion
Electrochemical Techniques - methods
electrochemistry
HEK293 Cells
Humans
Imaging
Microscopy
Microscopy - methods
oxygen
Oxygen Consumption
Respiration
Scanning
scanning probe microscopy
Visualization
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Summary:The influence of the reaction rate at the SECM tip on the overall imaging result is often neglected during respiration studies performed by SECM. The effect of the driving force of the tip reaction is elucidated using a potential pulse profile implemented into a constant‐distance mode. Time‐dependent data acquisition allows visualization of the transition between a tip behaving as a passive observer and a tip actively inducing transmembrane diffusion of oxygen.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201301098