Flow-Based Enzymatic Ligation by SortaseA

Sortase-mediated ligation (sortagging) is a versatile, powerful strategy for protein modification. Because the sortase reaction reaches equilibrium, a large excess of polyglycine nucleophile is often employed to drive the reaction forward and suppress sortase-mediated side reactions. A flow-based so...

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Bibliographic Details
Published in:Angewandte Chemie 2014-08, Vol.126 (35), p.9357-9362
Main Authors: Policarpo, Rocco L, Kang, Hansol, Liao, Xiaoli, Rabideau, Amy E, Simon, Mark D, Pentelute, Bradley L
Format: Article
Language:eng ; ger
Subjects:
Chemistry
Microreactors
Nucleophiles
Platforms
Proteins
Strategy
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Description
Summary:Sortase-mediated ligation (sortagging) is a versatile, powerful strategy for protein modification. Because the sortase reaction reaches equilibrium, a large excess of polyglycine nucleophile is often employed to drive the reaction forward and suppress sortase-mediated side reactions. A flow-based sortagging platform employing immobilized sortaseA within a microreactor was developed that permits efficient sortagging at low nucleophile concentrations. The platform was tested with several reaction partners and used to generate a protein bioconjugate inaccessible by solution-phase batch sortagging.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201403582