Imaging Mass Spectrometry and Genome Mining Reveal Highly Antifungal Virulence Factor of Mushroom Soft Rot Pathogen

Caught in the act: Imaging mass spectrometry of a button mushroom (see picture) infected with the soft rot pathogen Janthinobacterium agaricidamnosum in conjunction with genome mining revealed jagaricin as a highly antifungal virulence factor that is not produced under standard cultivation condition...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2012-12, Vol.51 (52), p.13173-13177
Main Authors: Graupner, Katharina, Scherlach, Kirstin, Bretschneider, Tom, Lackner, Gerald, Roth, Martin, Gross, Harald, Hertweck, Christian
Format: Article
Language:English
Subjects:
Agaricales - growth & development
Antifungal Agents - chemistry
Antifungal Agents - metabolism
Antifungal Agents - pharmacology
biosynthesis
Buttons
Computational Biology
Fungi - drug effects
Genome, Bacterial
Genomes
Gram-Negative Bacteria - genetics
Gram-Negative Bacteria - isolation & purification
Imaging
Janthinobacterium agaricidamnosum
lipopeptides
Magnetic Resonance Spectroscopy
Mass spectrometry
Mining
Molecular Conformation
Mushrooms
natural products
Pathogens
Peptide Synthases - genetics
Peptide Synthases - metabolism
Peptides, Cyclic - chemical synthesis
Peptides, Cyclic - chemistry
Peptides, Cyclic - pharmacology
Plant Roots - microbiology
Polyketide Synthases - genetics
Polyketide Synthases - metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
structure elucidation
Virulence
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Summary:Caught in the act: Imaging mass spectrometry of a button mushroom (see picture) infected with the soft rot pathogen Janthinobacterium agaricidamnosum in conjunction with genome mining revealed jagaricin as a highly antifungal virulence factor that is not produced under standard cultivation conditions. The structure of jagaricin was rigorously elucidated by a combination of physicochemical analyses, chemical derivatization, and bioinformatics.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201206658