An RNA Splicing Enhancer that Does Not Act by Looping

Out of the loop: Do the proteins bound to an enhancer site on pre‐mRNA interact directly with the splice site by diffusion (looping), as is generally accepted, or does the intervening RNA play a role (see scheme)? By inserting a PEG linker between an enhancer sequence and alternative splice sites, t...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2012-09, Vol.51 (39), p.9800-9803
Main Authors: Lewis, Helen, Perrett, Andrew J., Burley, Glenn A., Eperon, Ian C.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Click Chemistry
Diffusion
Enhancer Elements, Genetic
Humans
Joints
mRNA
oligonucleotides
Oligonucleotides - chemistry
Oligonucleotides - genetics
Oligonucleotides - metabolism
pre-mRNA splicing
Proteins
Ribonucleic acids
RNA Precursors - chemistry
RNA Precursors - genetics
RNA Precursors - metabolism
RNA Splice Sites
RNA Splicing
RNA, Messenger - chemistry
RNA, Messenger - genetics
RNA, Messenger - metabolism
Splicing
splicing enhancers
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Summary:Out of the loop: Do the proteins bound to an enhancer site on pre‐mRNA interact directly with the splice site by diffusion (looping), as is generally accepted, or does the intervening RNA play a role (see scheme)? By inserting a PEG linker between an enhancer sequence and alternative splice sites, the interaction of these two elements can be studied. Intervening RNA was essential for the enhancer activity, which rules out the looping model.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201202932