Magnetobiosensors Based on Viral Protein p19 for MicroRNA Determination in Cancer Cells and Tissues

MicroRNAs (miRs) have emerged as important clinical biomarkers with both diagnostic and prognostic value for relevant diseases, such as cancer. MiRs pose unique challenges for detection and are currently detected by northern blotting, real‐time PCR, and microarray techniques. These expensive, compli...

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Bibliographic Details
Published in:Angewandte Chemie 2014-06, Vol.126 (24), p.6282-6285
Main Authors: Campuzano, Susana, Torrente-Rodríguez, Rebeca M., López-Hernández, Eva, Conzuelo, Felipe, Granados, Rosario, Sánchez-Puelles, José M., Pingarrón, José M.
Format: Article
Language:eng ; ger
Subjects:
Amperometrische Sensoren
Amplification
Biomarkers
Breast cancer
Cancer
Chemistry
Diagnostic systems
Electrical measurements
Human
Krebsdiagnostik
Magnetsensoren
Medical research
MikroRNA
Preprocessing
Proteine
Proteins
Ribonucleic acids
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Summary:MicroRNAs (miRs) have emerged as important clinical biomarkers with both diagnostic and prognostic value for relevant diseases, such as cancer. MiRs pose unique challenges for detection and are currently detected by northern blotting, real‐time PCR, and microarray techniques. These expensive, complicated, and time‐consuming techniques are not feasible for on‐site miR determination. In this study, amperometric magnetobiosensors involving RNA‐binding viral protein p19 as a selective biorecognition element were developed for miR quantification. The p19‐based magnetosensors were able to detect 0.4 fmol of a synthetic target and endogenous miR‐21 (selected as a model for its role in a wide variety of cancers) in only 2 h in total RNA extracted from cancer cells and human breast‐tumor specimens without PCR amplification and sample preprocessing. These results open up formidable perspectives for the diagnosis and prognosis of human cancers and for drug‐discovery programs. Ein amperometrischer Magnetsensor basierend auf einer spezifischen RNA‐Sonde (rote Stränge) und Protein p19 als Erkennungselemente wurde als Einweg‐Diagnoseinstrument für die Bestimmung von miR‐21 (grüne Stränge) in Krebszellen und ‐gewebe entwickelt. Durch Markieren des biotinylierten Hybrids mit an Meerrettichperoxidase konjugiertem Streptavidin (Strep–HRP) gelang der elektrochemische Nachweis auf einer aufgedruckten Kohlenstoffelektrode.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201403270