Acidic-pH-Activatable Fluorescence Probes for Visualizing Exocytosis Dynamics

Live imaging of exocytosis dynamics is crucial for a precise spatiotemporal understanding of secretion phenomena, but current approaches have serious limitations. We designed and synthesized small‐molecular fluorescent probes that were chemically optimized for sensing acidic intravesicular pH values...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2014-06, Vol.53 (24), p.6085-6089
Main Authors: Asanuma, Daisuke, Takaoka, Yousuke, Namiki, Shigeyuki, Takikawa, Kenji, Kamiya, Mako, Nagano, Tetsuo, Urano, Yasuteru, Hirose, Kenzo
Format: Article
Language:English
Subjects:
acidity
Cellular
Dynamic tests
Dynamics
exocytosis
Exocytosis - physiology
Fluorescence
Fluorescent Dyes - chemistry
fluorescent probes
Hydrogen-Ion Concentration
Imaging
Secretions
spatiotemporal dynamics
Stimulation
vesicular imaging
Visualization
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Summary:Live imaging of exocytosis dynamics is crucial for a precise spatiotemporal understanding of secretion phenomena, but current approaches have serious limitations. We designed and synthesized small‐molecular fluorescent probes that were chemically optimized for sensing acidic intravesicular pH values, and established that they can be used to sensitively and reliably visualize vesicular dynamics following stimulation. This straightforward technique for the visualization of exocytosis as well as endocytosis/reacidification processes with high spatiotemporal precision is expected to be a powerful tool for investigating dynamic cellular phenomena involving changes in the pH value. Going live: Live imaging of the dynamics of exocytosis is crucial for a precise spatiotemporal understanding of secretion phenomena. Small‐molecular fluorescent probes designed to be activated under acidic intravesicular conditions enabled the sensitive and reliable visualization of vesicular dynamics with high spatiotemporal precision (see picture).
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201402030