Protein Expression, Aggregation, and Triggered Release from Polymersomes as Artificial Cell-like Structures

Bringing droplets to life: A cytoskeletal protein (red dots, see scheme) is expressed in artificial cells composed of biocompatible polymersomes, which encapsulate expression machinery and amino acid building blocks. Release of the expressed proteins can be triggered by a negative osmotic shock.

Saved in:
Bibliographic Details
Published in:Angewandte Chemie International Edition 2012-06, Vol.51 (26), p.6416-6420
Main Authors: Martino, Chiara, Kim, Shin-Hyun, Horsfall, Louise, Abbaspourrad, Alireza, Rosser, Susan J., Cooper, Jonathan, Weitz, David A.
Format: Article
Language:English
Subjects:
Agglomeration
Amino acids
Artificial Cells - chemistry
Biocompatibility
double emulsions
Droplets
Encapsulation
Machinery
MreB
Oils - chemistry
Osmotic Pressure
polymersomes
protein expression
Proteins
Proteins - chemistry
synthetic biology
Water - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bringing droplets to life: A cytoskeletal protein (red dots, see scheme) is expressed in artificial cells composed of biocompatible polymersomes, which encapsulate expression machinery and amino acid building blocks. Release of the expressed proteins can be triggered by a negative osmotic shock.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201201443