Protein Expression, Aggregation, and Triggered Release from Polymersomes as Artificial Cell-like Structures

Bringing droplets to life: A cytoskeletal protein (red dots, see scheme) is expressed in artificial cells composed of biocompatible polymersomes, which encapsulate expression machinery and amino acid building blocks. Release of the expressed proteins can be triggered by a negative osmotic shock.

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Published in:Angewandte Chemie International Edition 2012-06, Vol.51 (26), p.6416-6420
Main Authors: Martino, Chiara, Kim, Shin-Hyun, Horsfall, Louise, Abbaspourrad, Alireza, Rosser, Susan J., Cooper, Jonathan, Weitz, David A.
Format: Article
Language:English
Subjects:
Agglomeration
Amino acids
Artificial Cells - chemistry
Biocompatibility
double emulsions
Droplets
Encapsulation
Machinery
MreB
Oils - chemistry
Osmotic Pressure
polymersomes
protein expression
Proteins
Proteins - chemistry
synthetic biology
Water - chemistry
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cited_by cdi_FETCH-LOGICAL-c5103-d90bf8ebc91c715727be792305ed355725ca92069225249960fb447690fadb913
cites cdi_FETCH-LOGICAL-c5103-d90bf8ebc91c715727be792305ed355725ca92069225249960fb447690fadb913
container_end_page 6420
container_issue 26
container_start_page 6416
container_title Angewandte Chemie International Edition
container_volume 51
creator Martino, Chiara
Kim, Shin-Hyun
Horsfall, Louise
Abbaspourrad, Alireza
Rosser, Susan J.
Cooper, Jonathan
Weitz, David A.
description Bringing droplets to life: A cytoskeletal protein (red dots, see scheme) is expressed in artificial cells composed of biocompatible polymersomes, which encapsulate expression machinery and amino acid building blocks. Release of the expressed proteins can be triggered by a negative osmotic shock.
doi_str_mv 10.1002/anie.201201443
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subjects Agglomeration
Amino acids
Artificial Cells - chemistry
Biocompatibility
double emulsions
Droplets
Encapsulation
Machinery
MreB
Oils - chemistry
Osmotic Pressure
polymersomes
protein expression
Proteins
Proteins - chemistry
synthetic biology
Water - chemistry
title Protein Expression, Aggregation, and Triggered Release from Polymersomes as Artificial Cell-like Structures
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