Template-Directed Synthesis in 3′- and 5′-Direction with Reversible Termination

Extending both ways: A method for DNA‐templated synthesis on solid support is described. Controlled, stepwise chain extension was demonstrated both in the direction favored by nature (3′‐extension; see scheme) and in the direction typical for conventional DNA synthesizers (5′‐extension).

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2012-08, Vol.51 (33), p.8299-8303
Main Authors: Kaiser, Andreas, Spies, Sebastian, Lommel, Tanja, Richert, Clemens
Format: Article
Language:English
Subjects:
Base Sequence
Chains
Deoxyribonucleic acid
DNA
DNA - chemical synthesis
DNA - chemistry
primer extension
protecting groups
reversible terminator
solid-phase synthesis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Synthesis
Synthesizers
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Description
Summary:Extending both ways: A method for DNA‐templated synthesis on solid support is described. Controlled, stepwise chain extension was demonstrated both in the direction favored by nature (3′‐extension; see scheme) and in the direction typical for conventional DNA synthesizers (5′‐extension).
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201203859