Nanostructured silver-gold bimetallic SERS substrates for selective identification of bacteria in human blood

Surface-enhanced Raman spectroscopy (SERS) is a potentially important tool in the rapid and accurate detection of pathogenic bacteria in biological fluids. However, for diagnostic application of this technique, it is necessary to develop a highly sensitive, stable, biocompatible and reproducible SER...

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Bibliographic Details
Published in:Analyst (London) 2014-01, Vol.139 (5), p.1037-1043
Main Authors: Sivanesan, Arumugam, Witkowska, Evelin, Adamkiewicz, Witold, Dziewit, Łukasz, Kamińska, Agnieszka, Waluk, Jacek
Format: Article
Language:English
Subjects:
Antibiotics
Atmospherics
Bacillus megaterium
Bacteria
Bimetals
Blood
Escherichia coli
Escherichia coli - isolation & purification
Escherichia coli - metabolism
Gold - chemistry
Gold - metabolism
Humans
Metal Nanoparticles - chemistry
Nanostructure
Silver
Silver - chemistry
Silver - metabolism
Spectrum Analysis, Raman - methods
Staphylococcus epidermidis - isolation & purification
Staphylococcus epidermidis - metabolism
Substrate Specificity - physiology
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Summary:Surface-enhanced Raman spectroscopy (SERS) is a potentially important tool in the rapid and accurate detection of pathogenic bacteria in biological fluids. However, for diagnostic application of this technique, it is necessary to develop a highly sensitive, stable, biocompatible and reproducible SERS-active substrate. In this work, we have developed a silver-gold bimetallic SERS surface by a simple potentiostatic electrodeposition of a thin gold layer on an electrochemically roughened nanoscopic silver substrate. The resultant substrate was very stable under atmospheric conditions and exhibited the strong Raman enhancement with the high reproducibility of the recorded SERS spectra of bacteria (E. coli, S. enterica, S. epidermidis, and B. megaterium). The coating of the antibiotic over the SERS substrate selectively captured bacteria from blood samples and also increased the Raman signal in contrast to the bare surface. Finally, we have utilized the antibiotic-coated hybrid surface to selectively identify different pathogenic bacteria, namely E. coli, S. enterica and S. epidermidis from blood samples.
ISSN:0003-2654
1364-5528
DOI:10.1039/c3an01924a