Isolation, structure elucidation and enzyme inhibition studies of a new hydroxy ester and other compounds from Berberis jaeschkeana Schneid stem

• Published in: Natural product research 2015-09, Vol.29 (17), p.1664-1669
• Main Authors: Alamzeb, Muhammad, Khan, M. Rafiullah, Mamoon-Ur-Rashid, Ali, Saqib, Khan, Ashfaq Ahmad
• Format: Article
• Language: English
• Subjects: Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; berberinol; Berberis - chemistry; Berberis jaeschkeana; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - isolation & purification; Esters - chemistry; Esters - isolation & purification; Heterocyclic Compounds, 4 or More Rings - chemistry; Heterocyclic Compounds, 4 or More Rings - isolation & purification; Microbial Sensitivity Tests; Molecular Structure; Plant Extracts - chemistry; Plant Stems - chemistry; Urease - antagonists & inhibitors; urease enzyme
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2014.1000321

Bioassay-guided isolation and fractionation of Berberis jaeschkeana Schneid var. jaeschkeana stem resulted in the isolation and characterisation of a new long chain hydroxy ester named as berberinol (1) along with six known compounds (2-7). All the structures were established from 1D and 2D spectroscopic data. Crude extract, sub-fractions and all the isolated compounds were evaluated for their anti-fungal and urease enzyme inhibition properties. All of the sub-fractions and compounds showed good anti-fungal and urease enzyme inhibition properties. Minimum inhibitory concentrations (MICs) were calculated for all active samples in case of urease enzyme inhibition. MICs values were found to be in the range of 39.03-49.78 μg/mL for urease enzyme inhibition.