Differential expression of microRNAs in renal transplant patients with acute T-cell mediated rejection

• Published in: Transplant immunology 2015-09, Vol.33 (1), p.1-6
• Main Authors: Soltaninejad, Ehsan, Nicknam, Mohammad Hossein, Nafar, Mohsen, Ahmadpoor, Pedram, Pourrezagholi, Fatemeh, Sharbafi, Mohammad Hossein, Hosseinzadeh, Morteza, Foroughi, Farshad, Yekaninejad, Mir Saeed, Bahrami, Tayyeb, Sharif-Paghaleh, Ehsan, Amirzargar, Aliakbar
• Format: Article
• Language: English
• Subjects: Acute Disease; Acute T-cell mediated rejection; Adult; Aged; Allergy and Immunology; Biomarker; Biopsy; Female; Gene Expression Regulation - immunology; Graft Rejection - diagnosis; Graft Rejection - immunology; Graft Rejection - pathology; Humans; Kidney - immunology; Kidney - pathology; Kidney Transplantation; Male; MicroRNA; MicroRNAs - immunology; Middle Aged; Renal transplantation; T-Lymphocytes - immunology; T-Lymphocytes - pathology
• ISSN: 0966-3274; 1878-5492
• DOI: 10.1016/j.trim.2015.05.002

Abstract Background MicroRNAs (miRNAs) regulate most of encoding genes and protein. In this study, we aimed to investigate the expression levels of miR-142-5p, miR-142-3p, miR-155 and miR-223 in paired biopsy and peripheral blood mononuclear cell (PBMC) samples of renal allograft recipients with acute T-cell mediated rejection (ATCMR), compared with normal allografts (NA). Methods In this study, the expression levels of individual miRNAs were determined in biopsy and PBMC samples of 17 recipients with ATCMR and 18 recipients with NA. Results Our results showed that the intragraft expression levels of all studied miRNAs were significantly higher in ATCMR than NA. However, regarding the PBMC samples, miR-142-3p and miR-223 were significantly increased in ATCMR than NA. Receiver operating characteristic (ROC) analysis showed that miR-142-5p, miR-142-3p, miR-155 and miR-223 in biopsy samples and miR-142-3p and miR-223 in PBMC samples could discriminate ATCMR from NA recipients. Conclusion It has been reported that high intragraft expressions of miRNAs have a profound role in the pathogenesis of ATCMR process. Our results showed that high expression of all the studied miRNAs in biopsies and miR-142-3p and miR-223 in PBMC samples could be used as suggestive diagnostic tools to discriminate ATCMR patients from NA.