Neuronal tumour necrosis factor-α and interleukin-1β expression in a porcine model of intracerebral haemorrhage: Modulation by U-74389G

• Published in: Brain research 2015-07, Vol.1615, p.98-105
• Main Authors: Bimpis, Alexios, Papalois, Apostolos, Voumvourakis, Konstantinos, Oláh, Orsolya, Tiszlavicz, Lazlo, Liapi, Charis
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - administration & dosage; Basal Ganglia - drug effects; Basal Ganglia - metabolism; Cerebral Hemorrhage - metabolism; Cerebral Hemorrhage - prevention & control; IL-1β; Interleukin-1beta - metabolism; Intracerebral haemorrhage; Male; Neurology; Neurons - drug effects; Neurons - metabolism; Porcine model; Pregnatrienes - administration & dosage; Swine; TNF-α; Tumor Necrosis Factor-alpha - metabolism; U-74389G
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2015.04.034

Abstract Tumour necrosis factor α (TNF-α) and interleukin 1β (IL-1β) are important mediators of intracerebral haemorrhage (ICH) inflammatory response. Lazaroids, established antioxidants and neuroprotectants, have been studied in several brain pathologies. The present study was designed to investigate: a) TNF-α and IL-1β changes, in neurons and b) U-74389G effects, 4 and 24 h after haematoma induction in a porcine model of intracerebral haemorrhage. In twenty male landrace pigs (swines) aged 135–150 days old, autologous whole blood was injected around the right basal ganglia territory; in ten of the pigs the lazaroid compound U-74389G was administered. Brain TNF-α and IL-1β immunopositive neurons were determined by immunoarray techniques at 4 and 24 h timepoints. After the haematoma induction the number of TNF-α immunopositive neurons ipsilateral to the haematoma was significantly higher compared to the contralateral site at 4 h ( p