Lysine methylation modulates the protein-protein interactions of yeast cytochrome C Cyc1p

In recent years, protein methylation has been established as a major intracellular PTM. It has also been proposed to modulate protein‐protein interactions (PPIs) in the interactome. To investigate the effect of PTMs on PPIs, we recently developed the conditional two‐hybrid (C2H) system. With this, w...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2015-07, Vol.15 (13), p.2166-2176
Main Authors: Winter, Daniel L., Abeygunawardena, Dhanushi, Hart-Smith, Gene, Erce, Melissa A., Wilkins, Marc R.
Format: Article
Language:English
Subjects:
Blotting, Western
Cytochrome
Cytochrome c
Cytochromes c - metabolism
Escherichia coli - metabolism
Lysine - metabolism
Lysine methylation
Mass Spectrometry
Methylation
Protein Binding
Protein-protein interactions
Proteins
Systems biology
Two-hybrid
Two-Hybrid System Techniques
Yeast
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Summary:In recent years, protein methylation has been established as a major intracellular PTM. It has also been proposed to modulate protein‐protein interactions (PPIs) in the interactome. To investigate the effect of PTMs on PPIs, we recently developed the conditional two‐hybrid (C2H) system. With this, we demonstrated that arginine methylation can modulate PPIs in the yeast interactome. Here, we used the C2H system to investigate the effect of lysine methylation. Specifically, we asked whether Ctm1p‐mediated trimethylation of yeast cytochrome c Cyc1p, on lysine 78, modulates its interactions with Erv1p, Ccp1p, Cyc2p and Cyc3p. We show that the interactions between Cyc1p and Erv1p, and between Cyc1p and Cyc3p, are significantly increased upon trimethylation of lysine 78. This increase of interaction helps explain the reported facilitation of Cyc1p import into the mitochondrial intermembrane space upon methylation. This first application of the C2H system to the study of methyllysine‐modulated interactions further confirms its robustness and flexibility.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.201400521