Differential effects of duration of sleep fragmentation on spatial learning and synaptic plasticity in pubertal mice

• Published in: Brain research 2015-07, Vol.1615, p.116-128
• Main Authors: Wallace, Eli, Kim, Do Young, Kim, Kye-Min, Chen, Stephanie, Blair Braden, B, Williams, Jeremy, Jasso, Kalene, Garcia, Alex, Rho, Jong M, Bimonte-Nelson, Heather, Maganti, Rama
• Format: Article
• Language: English
• Subjects: Adolescence; Animals; Cerebral Cortex - physiopathology; Corticosterone - blood; Electric Stimulation; Electroencephalography; Electromyography; Growth; Hippocampus - physiopathology; Long-Term Potentiation; LTP; Male; Memory - physiology; Mice; Mice, Inbred C57BL; Morris water maze; Motor Activity; Neurology; Sleep Deprivation - physiopathology; Sleep Deprivation - psychology; Sleep fragmentation; Spatial Learning - physiology; Spatial memory
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2015.04.037

Abstract Study objective To examine the differential effects of acute and chronic sleep fragmentation (SF) on spatial learning and memory, and hippocampal long-term potentiation (LTP) in pubertal mice. Methods Two studies were performed during which adolescent C57/Bl6 mice were subjected to acute-SF 24 h a day×3 days or chronic-SF for 12 h a day×2 weeks using a programmable rotating lever that provides tactile stimulus with controls housed in similar cages. Spatial learning and memory was examined using the Morris water maze, and long-term potentiation (LTP) was evaluated after stimulation of Schaffer collaterals in CA1 hippocampus post SF. Actigraphy was used during the period of SF to monitor rest-activity patterns. Electroencephalographic (EEG) recordings were acquired for analysis of vigilance state patterns and delta-power. Serum corticosterone was measured to assess stress levels. Results Acute-SF via tactile stimulation negatively impacted spatial learning, as well as LTP maintenance, compared to controls with no tactile stimulation. While actigraphy showed significantly increased motor activity during SF in both groups, EEG data indicated that overall sleep efficiency did not differ between baseline and SF days, but significant increases in number of wakeful bouts and decreases in average NREM and REM bout lengths were seen during lights-on. Acute sleep fragmentation did not impact corticosterone levels. Conclusions The current results indicate that, during development in pubertal mice, acute-SF for 24 h a day×3 days negatively impacted spatial learning and synaptic plasticity. Further studies are needed to determine if any inherent long-term homeostatic mechanisms in the adolescent brain afford greater resistance to the deleterious effects of chronic-SF.