Size control of in vitro synthesized magnetite crystals by the MamC protein of Magnetococcus marinus strain MC-1

• Published in: Applied microbiology and biotechnology 2015-06, Vol.99 (12), p.5109-5121
• Main Authors: Valverde-Tercedor, C, Montalbán-López, M, Perez-Gonzalez, T, Sanchez-Quesada, M. S, Prozorov, T, Pineda-Molina, E, Fernandez-Vivas, M. A, Rodriguez-Navarro, A. B, Trubitsyn, D, Bazylinski, Dennis A, Jimenez-Lopez, C
• Format: Article
• Language: English
• Subjects: Alphaproteobacteria - chemistry; Alphaproteobacteria - genetics; Alphaproteobacteria - metabolism; Analysis; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biomedical and Life Sciences; biomimetic synthesis; Biomimetics; Biotechnologically Relevant Enzymes and Proteins; Biotechnology; Crystals; Ferrosoferric Oxide - chemistry; Ferrosoferric Oxide - metabolism; Genes; lactalbumin; Life Sciences; lysozyme; Magnetism; Magnetite; Magnetococcus; Magnetosomes - chemistry; Magnetosomes - genetics; Magnetosomes - metabolism; Microbial Genetics and Genomics; Microbiological synthesis; Microbiology; Mineralization; Morphology; myoglobin; Myoglobins; Nanocrystals; Nanoparticles; Particle Size; Physiological aspects; Production processes; Prokaryotes; prokaryotic cells; Properties; Proteins; recombinant proteins; Studies
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-014-6326-y

Magnetotactic bacteria are a diverse group of prokaryotes that share the unique ability of biomineralizing magnetosomes, which are intracellular, membrane-bounded crystals of either magnetite (Fe₃O₄) or greigite (Fe₃S₄). Magnetosome biomineralization is mediated by a number of specific proteins, many of which are localized in the magnetosome membrane, and thus is under strict genetic control. Several studies have partially elucidated the effects of a number of these magnetosome-associated proteins in the control of the size of magnetosome magnetite crystals. However, the effect of MamC, one of the most abundant proteins in the magnetosome membrane, remains unclear. In this present study, magnetite nanoparticles were synthesized inorganically in free-drift experiments at 25 °C in the presence of different concentrations of the iron-binding recombinant proteins MamC and MamCnts (MamC without its first transmembrane segment) from the marine, magnetotactic bacterium Magnetococcus marinus strain MC-1 and three commercial proteins [α-lactalbumin (α-Lac), myoglobin (Myo), and lysozyme (Lyz)]. While no effect was observed on the size of magnetite crystals formed in the presence of the commercial proteins, biomimetic synthesis in the presence of MamC and MamCnts at concentrations of 10–60 μg/mL resulted in the production of larger and more well-developed magnetite crystals (~30–40 nm) compared to those of the control (~20–30 nm; magnetite crystals grown protein-free). Our results demonstrate that MamC plays an important role in the control of the size of magnetite crystals and could be utilized in biomimetic synthesis of magnetite nanocrystals.