Interesterified fat or palm oil as substitutes for partially hydrogenated fat during the perinatal period produces changes in the brain fatty acids profile and increases leukocyte–endothelial interactions in the cerebral microcirculation from the male offspring in adult life

• Published in: Brain research 2015-08, Vol.1616, p.123-133
• Main Authors: Misan, Vanessa, Estato, Vanessa, de Velasco, Patricia Coelho, Spreafico, Flavia Brasil, Magri, Tatiana, dos Santos, Raísa Magno de Araújo Ramos, Fragoso, Thaiza, Souza, Amanda S, Boldarine, Valter Tadeu, Bonomo, Isabela T, Sardinha, Fátima L.C, Oyama, Lila M, Tibiriçá, Eduardo, Tavares do Carmo, Maria das Graças
• Format: Article
• Language: English
• Subjects: Age Factors; Analysis of Variance; Animals; Animals, Newborn; Body Weight; Brain - anatomy & histology; Brain - growth & development; Brain - metabolism; Brain microcirculation; Diet; Dietary lipid; Eating; Endothelium - metabolism; Fatty Acids - metabolism; Female; Humans; Inflammation; Interesterified fat; Leukocytes - metabolism; Male; Metabolic programming; Mice; Mice, Inbred C57BL; Microcirculation - physiology; Neurology; Palm Oil; Plant Oils - administration & dosage; Pregnancy; Prenatal Exposure Delayed Effects; Soybean Oil - administration & dosage; Toll-Like Receptor 4 - metabolism; Trans Fatty Acids - administration & dosage
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2015.05.001

Abstract We investigated whether maternal intake of normolipidic diets with distinct fatty acid (FA) compositions alters the lipidic profile and influences the inflammatory status of the adult offsprings׳ brains. C57BL/6 female mice during pregnancy and lactation received diets containing either soybean oil (CG), partially hydrogenated vegetable fat rich in trans -fatty acids (TG), palm oil (PG), or interesterified fat (IG). After weaning, male offspring from all groups received control diet. The FA profile was measured in the offspring׳s brains at post-natal days 21 and 90. Brain functional capillary density as well as leukocyte–endothelial interactions in the cerebral post-capillary venules was assessed by intravital fluorescence microscopy at post-natal day 90. Inflammation signaling was evaluated through toll-like receptor 4 (TLR4) content in brain of the adult offspring. In the 21-day old offspring, the brains of the TG showed higher levels of trans FA and reduced levels of linoleic acid (LA) and total n -6 polyunsaturated fatty acids (PUFA). At post-natal day 90, TG and IG groups showed reduced levels of eicosapentaenoic acid (EPA) and total n -3 PUFA tended to be lower compared to CG. The offspring׳s brains exhibited an altered microcirculation with increased leukocyte rolling in groups TG, PG and IG and in TG group increased leukocyte adhesion. The TLR4 content of TG, IG and PG groups only tended to increase (23%; 20% and 35%, respectively). Maternal consumption of trans FA, palm oil or interesterified fat during pregnancy and lactation can trigger the initial steps of inflammatory pathways in the brain of offspring in adulthood.