Effect of cloned Salmonella typhimurium enterotoxin on rabbit intestinal motility

We analysed the small intestine myoelectric responses of anesthetized New Zealand albino rabbits to Escherichia coli lysates containing an enterotoxin cloned from Salmonella typhimurium. Migrating action potential complex, which consisted of rapid bursts of action potentials and secretion of fluid,...

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Bibliographic Details
Published in:FEMS microbiology letters 1995-12, Vol.134 (2‐3), p.239-244
Main Authors: Reeves‐Darby, Vonda G., Turner, John A., Prasad, Rajendra, Chopra, Ashok K., Chary, Parvathi, Clench, Mary H., Peterson, Johnny W., Mathias, John R.
Format: Article
Language:English
Subjects:
Action Potentials - drug effects
Animals
Bacteriology
Biological and medical sciences
Cholera toxin
Cloning, Molecular
Enterotoxins - genetics
Enterotoxins - toxicity
Fundamental and applied biological sciences. Psychology
Gastrointestinal Motility - drug effects
Ileum - drug effects
Ileum - physiology
Ileum - secretion
Male
Microbiology
Migration action potential complex
Myoelectric Complex, Migrating - drug effects
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Rabbits
Recombinant Proteins - genetics
Recombinant Proteins - toxicity
Salmonella Infections, Animal - etiology
Salmonella Infections, Animal - physiopathology
Salmonella typhimurium
Salmonella typhimurium - genetics
Salmonella typhimurium - pathogenicity
Stn
stn gene
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Summary:We analysed the small intestine myoelectric responses of anesthetized New Zealand albino rabbits to Escherichia coli lysates containing an enterotoxin cloned from Salmonella typhimurium. Migrating action potential complex, which consisted of rapid bursts of action potentials and secretion of fluid, was observed only in ileal loops injected with the enterotoxin‐containing lysate. Migrating action potential complex produced by Stn usually propagated aborally, which was typical of cholera toxin, but orad or bidirectional propagation occurred from a single point of origin when activity was intense. Gell lysates from an E. coli clone containing vectors alone, as well as proximal control segments injected with phosphate‐buffered saline, gave neither a change in motility nor fluid secretion. These results show that Stn caused dramatic changes in intestinal motility and substantial fluid production.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1995.tb07944.x