new monoclonal antibody detecting ochratoxin A at the picogram level

Á. GYÖNGYÖSI‐HORVÁTH, I. BARNA‐VETRÓ AND L. SOLTI. 1996. A monoclonal antibody against ochratoxin A was produced after immunization of Balb/c mice with ochratoxin A‐BSA. This antibody was of the IgGl heavy chain subclass with a k type light chain. The 50% inhibition value was 0.45 ng ml‐1 in a direc...

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Bibliographic Details
Published in:Letters in applied microbiology 1996-02, Vol.22 (2), p.103-105
Main Authors: Gyongyosi-Horvath, A, Barna-Vetro, I, Solti, L
Format: Article
Language:English
Subjects:
Animals
Antibodies, immunoglobulins
Antibodies, Monoclonal - biosynthesis
Antibody Specificity
Biological and medical sciences
cross reaction
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - standards
Enzyme-Linked Immunosorbent Assay - statistics & numerical data
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Hybridomas - immunology
Immunization
Immunoglobulin G - biosynthesis
Mice
Mice, Inbred BALB C
Molecular immunology
Monoclonal antibodies
Mycotoxins - analysis
Mycotoxins - immunology
Mycotoxins - standards
ochratoxins
Ochratoxins - analysis
Ochratoxins - immunology
Ochratoxins - standards
Reference Standards
Sensitivity and Specificity
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Summary:Á. GYÖNGYÖSI‐HORVÁTH, I. BARNA‐VETRÓ AND L. SOLTI. 1996. A monoclonal antibody against ochratoxin A was produced after immunization of Balb/c mice with ochratoxin A‐BSA. This antibody was of the IgGl heavy chain subclass with a k type light chain. The 50% inhibition value was 0.45 ng ml‐1 in a direct competitive ELISA and the detection limit was 42 pg ml‐1. This antibody is very specific, cross‐reacting only with ochratoxin B (9.3%).
ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.1996.tb01119.x