Detection of phialoconidia of Trichoderma harzianum in peat-bran by monoclonal antibody-based enzyme-linked immunosorbent assay

Murine monoclonal antibodies (MAbs) were raised against phialoconidia of a Trichoderma harzianum isolate T95. A hybridoma cell line, GH5, was raised that produced MAbs of the immunoglobulin class IgM that recognized, by enzyme-linked immunosorbent assay (ELISA), antigens from conidia of T95. They di...

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Bibliographic Details
Published in:Mycological research 1996, Vol.100 (2), p.217-222
Main Authors: Thornton, C.R., Dewey, F.M.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological control
Control
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Microbiology
Mycological methods and techniques used in mycology
Mycology
Phytopathology. Animal pests. Plant and forest protection
Trichoderma harzianum
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Summary:Murine monoclonal antibodies (MAbs) were raised against phialoconidia of a Trichoderma harzianum isolate T95. A hybridoma cell line, GH5, was raised that produced MAbs of the immunoglobulin class IgM that recognized, by enzyme-linked immunosorbent assay (ELISA), antigens from conidia of T95. They did not react with antigens from mycelium or chlamydospores of this isolate but recognized antigens from conidia of a number of other T. harzianum and T. viride isolates. Heat, protease and periodate treatment of T95 conidial antigens showed that GH5 MAbs recognized a protein epitope that formed part of a larger glycoprotein molecule. GH5 MAbs were used to develop an ELISA for the detection of phialoconidia of T. harzianum in peat-bran.
ISSN:0953-7562
1469-8102
DOI:10.1016/S0953-7562(96)80126-2