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Detection of phialoconidia of Trichoderma harzianum in peat-bran by monoclonal antibody-based enzyme-linked immunosorbent assay
Murine monoclonal antibodies (MAbs) were raised against phialoconidia of a Trichoderma harzianum isolate T95. A hybridoma cell line, GH5, was raised that produced MAbs of the immunoglobulin class IgM that recognized, by enzyme-linked immunosorbent assay (ELISA), antigens from conidia of T95. They di...
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| Published in: | Mycological research 1996, Vol.100 (2), p.217-222 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c367t-518bb38e12338c454b34a7c10745d93270f776c8e512c47e47bb10113981c8143 |
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| cites | cdi_FETCH-LOGICAL-c367t-518bb38e12338c454b34a7c10745d93270f776c8e512c47e47bb10113981c8143 |
| container_end_page | 222 |
| container_issue | 2 |
| container_start_page | 217 |
| container_title | Mycological research |
| container_volume | 100 |
| creator | Thornton, C.R. Dewey, F.M. |
| description | Murine monoclonal antibodies (MAbs) were raised against phialoconidia of a
Trichoderma harzianum isolate T95. A hybridoma cell line, GH5, was raised that produced MAbs of the immunoglobulin class IgM that recognized, by enzyme-linked immunosorbent assay (ELISA), antigens from conidia of T95. They did not react with antigens from mycelium or chlamydospores of this isolate but recognized antigens from conidia of a number of other
T. harzianum and
T. viride isolates. Heat, protease and periodate treatment of T95 conidial antigens showed that GH5 MAbs recognized a protein epitope that formed part of a larger glycoprotein molecule. GH5 MAbs were used to develop an ELISA for the detection of phialoconidia of
T. harzianum in peat-bran. |
| doi_str_mv | 10.1016/S0953-7562(96)80126-2 |
| format | article |
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Trichoderma harzianum isolate T95. A hybridoma cell line, GH5, was raised that produced MAbs of the immunoglobulin class IgM that recognized, by enzyme-linked immunosorbent assay (ELISA), antigens from conidia of T95. They did not react with antigens from mycelium or chlamydospores of this isolate but recognized antigens from conidia of a number of other
T. harzianum and
T. viride isolates. Heat, protease and periodate treatment of T95 conidial antigens showed that GH5 MAbs recognized a protein epitope that formed part of a larger glycoprotein molecule. GH5 MAbs were used to develop an ELISA for the detection of phialoconidia of
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Trichoderma harzianum isolate T95. A hybridoma cell line, GH5, was raised that produced MAbs of the immunoglobulin class IgM that recognized, by enzyme-linked immunosorbent assay (ELISA), antigens from conidia of T95. They did not react with antigens from mycelium or chlamydospores of this isolate but recognized antigens from conidia of a number of other
T. harzianum and
T. viride isolates. Heat, protease and periodate treatment of T95 conidial antigens showed that GH5 MAbs recognized a protein epitope that formed part of a larger glycoprotein molecule. GH5 MAbs were used to develop an ELISA for the detection of phialoconidia of
T. harzianum in peat-bran.</description><subject>Biological and medical sciences</subject><subject>Biological control</subject><subject>Control</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Microbiology</subject><subject>Mycological methods and techniques used in mycology</subject><subject>Mycology</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Trichoderma harzianum</subject><issn>0953-7562</issn><issn>1469-8102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkU2LFDEQhoMoOK7-BCEHET1EU5100n0SWT9hwYPrOVTS1Uy0OxmTHqH34l-3Z2fZq6eiivetj6cYew7yDUgwb7_LvlXCtqZ51ZvXnYTGiOYB24E2vehANg_Z7l7ymD2p9aeUoADUjv39QAuFJebE88gP-4hTDjnFIeKpcF1i2OeByox8j-UmYjrOPCZ-IFyEL5i4X_mcUw5TTjhxTEv0eViFx0oDp3SzziSmmH5tWZznY8o1F09p4Vgrrk_ZoxGnSs_u4gX78enj9eUXcfXt89fL91ciKGMX0ULnveoIGqW6oFvtlUYbQFrdDr1qrBytNaGjFpqgLWnr_cYGVN9B6ECrC_by3PdQ8u8j1cXNsQaaJkyUj9WBldBZbTdhexaGkmstNLpDiTOW1YF0J9zuFrc7sXS9cbe4XbP5XtwNwBpwGjc0IdZ7s5LSaHna491ZRtuxfyIVV0OkFGiIZfuDG3L8z6B_UJmVdg</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Thornton, C.R.</creator><creator>Dewey, F.M.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>1996</creationdate><title>Detection of phialoconidia of Trichoderma harzianum in peat-bran by monoclonal antibody-based enzyme-linked immunosorbent assay</title><author>Thornton, C.R. ; Dewey, F.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-518bb38e12338c454b34a7c10745d93270f776c8e512c47e47bb10113981c8143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Biological and medical sciences</topic><topic>Biological control</topic><topic>Control</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>Microbiology</topic><topic>Mycological methods and techniques used in mycology</topic><topic>Mycology</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Trichoderma harzianum</topic><toplevel>online_resources</toplevel><creatorcontrib>Thornton, C.R.</creatorcontrib><creatorcontrib>Dewey, F.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Mycological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thornton, C.R.</au><au>Dewey, F.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of phialoconidia of Trichoderma harzianum in peat-bran by monoclonal antibody-based enzyme-linked immunosorbent assay</atitle><jtitle>Mycological research</jtitle><date>1996</date><risdate>1996</risdate><volume>100</volume><issue>2</issue><spage>217</spage><epage>222</epage><pages>217-222</pages><issn>0953-7562</issn><eissn>1469-8102</eissn><abstract>Murine monoclonal antibodies (MAbs) were raised against phialoconidia of a
Trichoderma harzianum isolate T95. A hybridoma cell line, GH5, was raised that produced MAbs of the immunoglobulin class IgM that recognized, by enzyme-linked immunosorbent assay (ELISA), antigens from conidia of T95. They did not react with antigens from mycelium or chlamydospores of this isolate but recognized antigens from conidia of a number of other
T. harzianum and
T. viride isolates. Heat, protease and periodate treatment of T95 conidial antigens showed that GH5 MAbs recognized a protein epitope that formed part of a larger glycoprotein molecule. GH5 MAbs were used to develop an ELISA for the detection of phialoconidia of
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| fulltext | fulltext |
| identifier | ISSN: 0953-7562 |
| ispartof | Mycological research, 1996, Vol.100 (2), p.217-222 |
| issn | 0953-7562 1469-8102 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17018747 |
| source | ScienceDirect Freedom Collection |
| subjects | Biological and medical sciences Biological control Control Fundamental and applied biological sciences. Psychology Fungal plant pathogens Microbiology Mycological methods and techniques used in mycology Mycology Phytopathology. Animal pests. Plant and forest protection Trichoderma harzianum |
| title | Detection of phialoconidia of Trichoderma harzianum in peat-bran by monoclonal antibody-based enzyme-linked immunosorbent assay |
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