An in vitro evaluation of the antibacterial activity of the non-volatile phenolic fraction from rosemary leaves

In this study, the aim was to evaluate the antimicrobial action of the non-volatile phenols of rosemary leaves against two Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and two Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis). Three extracts with d...

Full description

Saved in:
Bibliographic Details
Published in:Natural product research 2015-08, Vol.29 (16), p.1537-1544
Main Authors: Sacco, C., Bellumori, M., Santomauro, F., Donato, R., Capei, R., Innocenti, M., Mulinacci, N.
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemistry
antibacterial activity
carnosic acid
disc diffusion method
Escherichia coli
Escherichia coli - drug effects
flavonoids
MBC
Microbial Sensitivity Tests
Phenols - chemistry
Plant Extracts - chemistry
Plant Leaves - chemistry
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
rosmarinic acid
Rosmarinus
Rosmarinus - chemistry
Staphylococcus - drug effects
Staphylococcus aureus
Staphylococcus epidermidis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, the aim was to evaluate the antimicrobial action of the non-volatile phenols of rosemary leaves against two Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and two Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis). Three extracts with different phenolic compositions were tested. By the agar disc diffusion method, Gram-positive bacteria were more sensitive to the extracts, and S. epidermidis showed the highest inhibition zones. Overall, all the extracts tested by the broth dilution method showed higher activity than results from the agar disc diffusion method. The minimal bactericidal concentration values indicated that E. coli was the most susceptible strain. This study demonstrated that the flavonoidic fraction of rosemary leaves does not play a crucial role as antimicrobial agents against these microorganisms. The most active extract was characterised by the highest amount of non-volatile terpenoidic compounds.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2014.986728