Determination of glucose-fructose oxidoreductase activity in whole cells of Zymomonas mobilis

A method for the determination of glucose-fructose oxidoreductase (GFOR) activity in whole cells of Zymomonas mobilis is described. The K m and the theoretical V max for GFOR were 192 g glucose.l(-1) and 17 g gluconic acid.g(-1) cell.h(-1), respectively. The changes in enthalpy (31.1 kJ.mol(-1)), en...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 1996, Vol.12 (1), p.22-24
Main Authors: ERZINGER, G. S, SILVEIRA, M. M, VITOLO, M, JONAS, R
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
Miscellaneous
Mission oriented research
Zymomonas mobilis
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Summary:A method for the determination of glucose-fructose oxidoreductase (GFOR) activity in whole cells of Zymomonas mobilis is described. The K m and the theoretical V max for GFOR were 192 g glucose.l(-1) and 17 g gluconic acid.g(-1) cell.h(-1), respectively. The changes in enthalpy (31.1 kJ.mol(-1)), entropy (0.41 kJ.K(-1)), and Gibbs free energy (-97.5 kJ.mol(-1)) related to glucose to gluconic acid conversion were also determined.
ISSN:0959-3993
1573-0972
DOI:10.1007/BF00327793