Determination of glucose-fructose oxidoreductase activity in whole cells of Zymomonas mobilis

A method for the determination of glucose-fructose oxidoreductase (GFOR) activity in whole cells of Zymomonas mobilis is described. The K m and the theoretical V max for GFOR were 192 g glucose.l(-1) and 17 g gluconic acid.g(-1) cell.h(-1), respectively. The changes in enthalpy (31.1 kJ.mol(-1)), en...

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Published in:World journal of microbiology & biotechnology 1996, Vol.12 (1), p.22-24
Main Authors: ERZINGER, G. S, SILVEIRA, M. M, VITOLO, M, JONAS, R
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
Miscellaneous
Mission oriented research
Zymomonas mobilis
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description A method for the determination of glucose-fructose oxidoreductase (GFOR) activity in whole cells of Zymomonas mobilis is described. The K m and the theoretical V max for GFOR were 192 g glucose.l(-1) and 17 g gluconic acid.g(-1) cell.h(-1), respectively. The changes in enthalpy (31.1 kJ.mol(-1)), entropy (0.41 kJ.K(-1)), and Gibbs free energy (-97.5 kJ.mol(-1)) related to glucose to gluconic acid conversion were also determined.
doi_str_mv 10.1007/BF00327793
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subjects Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
Miscellaneous
Mission oriented research
Zymomonas mobilis
title Determination of glucose-fructose oxidoreductase activity in whole cells of Zymomonas mobilis
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