Using the comet and micronucleus assays for genotoxicity studies: A review

Abstract Physical, chemical and biological agents can act in the DNA, resulting in mutation involved in cancer. Thus, genotoxic tests are required by regulatory agencies in order to evaluate potential risk of cancer. Among these tests, the comet assay (CA) and micronucleus assay (MNA) are the most c...

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Bibliographic Details
Published in:Biomedicine & pharmacotherapy 2015-05, Vol.72, p.74-82
Main Authors: Araldi, Rodrigo Pinheiro, de Melo, Thatiana Corrêa, Mendes, Thais Biude, de Sá Júnior, Paulo Luiz, Nozima, Bruno Heidi Nakano, Ito, Eliana Tiemi, de Carvalho, Rodrigo Franco, de Souza, Edislane Barreiros, de Cassia Stocco, Rita
Format: Article
Language:English
Subjects:
Animals
Comet assay
Comet Assay - methods
Genotoxicity tests
Humans
Internal Medicine
Medical Education
Micronucleus assay
Micronucleus Tests - methods
Models, Biological
Mutagenesis
Mutagenesis - genetics
Mutagenicity Tests - methods
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Summary:Abstract Physical, chemical and biological agents can act in the DNA, resulting in mutation involved in cancer. Thus, genotoxic tests are required by regulatory agencies in order to evaluate potential risk of cancer. Among these tests, the comet assay (CA) and micronucleus assay (MNA) are the most commonly used. However, there are different protocols and recommendations already published. This is the first review, after the inclusion of CA in S2R1 guidance and OECD 489, which summarizes the main technical recommendations of both CA and MNA.
ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2015.04.004