Studies on the exopolysaccharide from Sphingomonas paucimobilis-GS1 : nutritional requirements and precursor-forming enzymes

Studies on the nutritional requirements for optimal exopolysaccharide (EPS) production by Sphingomonas paucimobilis-GS1, in a synthetic medium revealed sucrose (40 g/L) and glutamate (0.5 g/L) or KNO sub(3) (1 g/L) to be the most suitable carbon and nitrogen sources, respectively. Ammonium salts wer...

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Bibliographic Details
Published in:Current microbiology 1995-10, Vol.31 (4), p.234-238
Main Authors: ASHTAPUTRE, A. A, SHAH, A. K
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Microbiology
Sphingomonas paucimobilis
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Summary:Studies on the nutritional requirements for optimal exopolysaccharide (EPS) production by Sphingomonas paucimobilis-GS1, in a synthetic medium revealed sucrose (40 g/L) and glutamate (0.5 g/L) or KNO sub(3) (1 g/L) to be the most suitable carbon and nitrogen sources, respectively. Ammonium salts were unfavorable to EPS accumulation, and inorganic phosphate above 10 mM affected the polymer quality. Specific activities of the EPS precursor-forming enzymes, UDP glucose pyrophosphorylase (UDPGPP) and phosphoglucomutase (PGluM), were four to five times lower, whereas that of UDPglucose dehydrogenase (UDPGDH) was 15-20 times lower under media conditions not favoring EPS production than under conditions favoring EPS accumulation. The activity of hexokinase (HK), however, remained constant. Considerably lower specific activities of PGluM and UDPGPP were also detected in some of the non-mucoid mutants.
ISSN:0343-8651
1432-0991
DOI:10.1007/bf00298380