Comparison of alginate and Pesta for formulation of Phanerochaete chrysosporium

Alginate and wheat gluten ("Pesta") matrices were compared for the encapsulation of the white rot fungus Phanerochaete chrysosporium. "Pesta" granules were not successful for formulating P. chrysosporium although control granules made with Alternaria alternata yielded viable fung...

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Bibliographic Details
Published in:Biotechnology techniques 1996, Vol.10 (1), p.7-12
Main Authors: BENNETT, J. W, TURNER, A. J, LOOMIS, A. K, CONNICK, W. J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Immobilization of organelles and whole cells
Immobilization techniques
Methods. Procedures. Technologies
Phanerochaete chrysosporium
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Summary:Alginate and wheat gluten ("Pesta") matrices were compared for the encapsulation of the white rot fungus Phanerochaete chrysosporium. "Pesta" granules were not successful for formulating P. chrysosporium although control granules made with Alternaria alternata yielded viable fungal colonies; the gluten in wheat flour apparently inhibits growth of the white rot fungus. P. chrysosporium formulated in alginate with corncob grits or sawdust, and stored at room temperature, yielded over 50% viability of encapsulated mycelia after six months. Alginate encapsulation offers a promising technology for the delivery of white rot fungi to toxic waste sites.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00161076