Simultaneous overproduction of extracellular endoglucanase and intracellular β-glucosidase by genetically engineered Bacillus subtilis

Simultaneous overproduction of intracellular beta -glucosidase and extracellular endoglucanase was attempted by constructing two artificial operon systems comprising the beta -glucosidase-endoglucanase gene( beta E) or the endoglucanase- beta -glucosidase gene(E beta ) under the control of a strong...

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Bibliographic Details
Published in:Biotechnology letters 1995-10, Vol.17 (10), p.1115-1120
Main Authors: JEONG HYUN KIM, BAEK-RAK LEE, PACK, M. Y
Format: Article
Language:English
Subjects:
Bacillus subtilis
Biological and medical sciences
Biotechnology
Enzyme engineering
Fermentation
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Production of selected enzymes
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Summary:Simultaneous overproduction of intracellular beta -glucosidase and extracellular endoglucanase was attempted by constructing two artificial operon systems comprising the beta -glucosidase-endoglucanase gene( beta E) or the endoglucanase- beta -glucosidase gene(E beta ) under the control of a strong engineered promoter, BJ27U Delta 88 and expressing them in Bacillus subtilis DB104. Two artificial operon systems contained 30 bp or 5 bp gap between the termination codon of the upstream gene and the SD sequence of the downstream gene, respectively. These operon systems were expressed well in B. subtilis and overproduced the beta -glucosidase cell extract as well as the endoglucanase supernatant. The level of expression in the operon system was almost the same as that in a single expression system.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF00143113