Optimisation of CCL64-based bioassay for TGF-β

Transforming growth factor β (TGF-β) is released by a variety of cells and known to be involved in many different processes including the immune response, wound healing and carcinogenesis. As most experimental investigations have been based on quantitative analysis of TGF-β production using a bioass...

Full description

Saved in:
Bibliographic Details
Published in:Journal of immunological methods 1995-10, Vol.186 (2), p.267-274
Main Authors: Garrigue-Antar, Laure, Barbieux, Isabelle, Lieubeau, Blandine, Boisteau, Olivier, Grégoire, Marc
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Bioassay
Biological and medical sciences
Biological Assay - methods
Blood Physiological Phenomena
Carcinoma - metabolism
Cattle
Cell Line
Colonic Neoplasms - metabolism
Culture Media, Conditioned
Culture Media, Serum-Free - pharmacology
Cytokines - metabolism
Dose-Response Relationship, Drug
Epidermal Growth Factor - pharmacology
Epithelium - drug effects
Epithelium - metabolism
Fibroblast Growth Factor 2 - pharmacology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Growth Inhibitors - analysis
Growth Inhibitors - pharmacology
Humans
Immunobiology
Lung - cytology
Lung - drug effects
Lung - metabolism
Lymphokines, interleukins ( function, expression)
Mink
Preservation, Biological
Recombinant Proteins - pharmacology
Regulatory factors and their cellular receptors
Sensitivity and Specificity
Swine
Transforming Growth Factor beta - analysis
Transforming Growth Factor beta - pharmacology
Transforming growth factor β
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Transforming growth factor β (TGF-β) is released by a variety of cells and known to be involved in many different processes including the immune response, wound healing and carcinogenesis. As most experimental investigations have been based on quantitative analysis of TGF-β production using a bioassay, it seemed important to test the validity and limitations of this method. This paper analyses several parameters that may impair TGF-β quantification by bioassay. Recommendations are made concerning the influence of technical parameters and the presence of other cytokines (EGF and bFGF) commonly released by cultured cells to which the Mv1Lu mink lung epithelial cell line (CCL64) is sensitive.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(95)00151-Y