Isolation of uracil auxotrophs from Burkholderia cepacia ATCC 25416

K. LI AND T.P. WEST. 1995. Two uracil auxotrophs of the phytopathogen Burkholderia cepacia ATCC 25416, which is known to be involved in food spoilage, were isolated by a combination of ethylmethane sulphonate and D‐cycloserine counterselection. One mutant exhibited depressed orotate phosphoribosyltr...

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Bibliographic Details
Published in:Letters in applied microbiology 1995-11, Vol.21 (5), p.336-339
Main Authors: Li, K., West, T.P.
Format: Article
Language:English
Subjects:
Aspartate Carbamoyltransferase - metabolism
Bacteriology
Biological and medical sciences
Burkholderia cepacia
Burkholderia cepacia - enzymology
Burkholderia cepacia - metabolism
Cycloserine - metabolism
Dihydroorotase - metabolism
Ethyl Methanesulfonate
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Microbiology
Mutagenesis
Orotate Phosphoribosyltransferase - metabolism
Orotidine-5'-Phosphate Decarboxylase - metabolism
Pyrimidine Nucleotides - metabolism
Pyrimidines - biosynthesis
Uracil - metabolism
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Summary:K. LI AND T.P. WEST. 1995. Two uracil auxotrophs of the phytopathogen Burkholderia cepacia ATCC 25416, which is known to be involved in food spoilage, were isolated by a combination of ethylmethane sulphonate and D‐cycloserine counterselection. One mutant exhibited depressed orotate phosphoribosyltransferase activity while the other mutant lacked orotidine 5'‐monophosphate decarboxylase activity. Pyrimidine limitation of either auxotroph elevated aspartate transcarbamoylase and dihydroorotase activities by at least 1.5‐fold indicating that these pathway enzymes may be repressible by a uracil‐related compound in B. cepacia. Overall, regulation of de novo pyrimidine synthesis in the uracil auxotrophs of B. cepacia ATCC 25416 was observed.
ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.1995.tb01074.x