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Isolation of uracil auxotrophs from Burkholderia cepacia ATCC 25416

K. LI AND T.P. WEST. 1995. Two uracil auxotrophs of the phytopathogen Burkholderia cepacia ATCC 25416, which is known to be involved in food spoilage, were isolated by a combination of ethylmethane sulphonate and D‐cycloserine counterselection. One mutant exhibited depressed orotate phosphoribosyltr...

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Published in:	Letters in applied microbiology 1995-11, Vol.21 (5), p.336-339
Main Authors:	Li, K., West, T.P.
Format:	Article
Language:	English
Subjects:	Aspartate Carbamoyltransferase - metabolism Bacteriology Biological and medical sciences Burkholderia cepacia Burkholderia cepacia - enzymology Burkholderia cepacia - metabolism Cycloserine - metabolism Dihydroorotase - metabolism Ethyl Methanesulfonate Fundamental and applied biological sciences. Psychology Metabolism. Enzymes Microbiology Mutagenesis Orotate Phosphoribosyltransferase - metabolism Orotidine-5'-Phosphate Decarboxylase - metabolism Pyrimidine Nucleotides - metabolism Pyrimidines - biosynthesis Uracil - metabolism
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description	K. LI AND T.P. WEST. 1995. Two uracil auxotrophs of the phytopathogen Burkholderia cepacia ATCC 25416, which is known to be involved in food spoilage, were isolated by a combination of ethylmethane sulphonate and D‐cycloserine counterselection. One mutant exhibited depressed orotate phosphoribosyltransferase activity while the other mutant lacked orotidine 5'‐monophosphate decarboxylase activity. Pyrimidine limitation of either auxotroph elevated aspartate transcarbamoylase and dihydroorotase activities by at least 1.5‐fold indicating that these pathway enzymes may be repressible by a uracil‐related compound in B. cepacia. Overall, regulation of de novo pyrimidine synthesis in the uracil auxotrophs of B. cepacia ATCC 25416 was observed.
doi_str_mv	10.1111/j.1472-765X.1995.tb01074.x
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LI AND T.P. WEST. 1995. Two uracil auxotrophs of the phytopathogen Burkholderia cepacia ATCC 25416, which is known to be involved in food spoilage, were isolated by a combination of ethylmethane sulphonate and D‐cycloserine counterselection. One mutant exhibited depressed orotate phosphoribosyltransferase activity while the other mutant lacked orotidine 5'‐monophosphate decarboxylase activity. Pyrimidine limitation of either auxotroph elevated aspartate transcarbamoylase and dihydroorotase activities by at least 1.5‐fold indicating that these pathway enzymes may be repressible by a uracil‐related compound in B. cepacia. Overall, regulation of de novo pyrimidine synthesis in the uracil auxotrophs of B. cepacia ATCC 25416 was observed.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>DOI: 10.1111/j.1472-765X.1995.tb01074.x</identifier><identifier>PMID: 7576529</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Aspartate Carbamoyltransferase - metabolism ; Bacteriology ; Biological and medical sciences ; Burkholderia cepacia ; Burkholderia cepacia - enzymology ; Burkholderia cepacia - metabolism ; Cycloserine - metabolism ; Dihydroorotase - metabolism ; Ethyl Methanesulfonate ; Fundamental and applied biological sciences. Psychology ; Metabolism. Enzymes ; Microbiology ; Mutagenesis ; Orotate Phosphoribosyltransferase - metabolism ; Orotidine-5'-Phosphate Decarboxylase - metabolism ; Pyrimidine Nucleotides - metabolism ; Pyrimidines - biosynthesis ; Uracil - metabolism</subject><ispartof>Letters in applied microbiology, 1995-11, Vol.21 (5), p.336-339</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4296-4f4df2f3c77386ff4e6a7d29b385426456b581f0e717206fa2906f21f050b9383</citedby><cites>FETCH-LOGICAL-c4296-4f4df2f3c77386ff4e6a7d29b385426456b581f0e717206fa2906f21f050b9383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2929216$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7576529$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, K.</creatorcontrib><creatorcontrib>West, T.P.</creatorcontrib><title>Isolation of uracil auxotrophs from Burkholderia cepacia ATCC 25416</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>K. LI AND T.P. WEST. 1995. Two uracil auxotrophs of the phytopathogen Burkholderia cepacia ATCC 25416, which is known to be involved in food spoilage, were isolated by a combination of ethylmethane sulphonate and D‐cycloserine counterselection. One mutant exhibited depressed orotate phosphoribosyltransferase activity while the other mutant lacked orotidine 5'‐monophosphate decarboxylase activity. Pyrimidine limitation of either auxotroph elevated aspartate transcarbamoylase and dihydroorotase activities by at least 1.5‐fold indicating that these pathway enzymes may be repressible by a uracil‐related compound in B. cepacia. Overall, regulation of de novo pyrimidine synthesis in the uracil auxotrophs of B. cepacia ATCC 25416 was observed.</description><subject>Aspartate Carbamoyltransferase - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Burkholderia cepacia</subject><subject>Burkholderia cepacia - enzymology</subject><subject>Burkholderia cepacia - metabolism</subject><subject>Cycloserine - metabolism</subject><subject>Dihydroorotase - metabolism</subject><subject>Ethyl Methanesulfonate</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Mutagenesis</subject><subject>Orotate Phosphoribosyltransferase - metabolism</subject><subject>Orotidine-5'-Phosphate Decarboxylase - metabolism</subject><subject>Pyrimidine Nucleotides - metabolism</subject><subject>Pyrimidines - biosynthesis</subject><subject>Uracil - metabolism</subject><issn>0266-8254</issn><issn>1472-765X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNqVkF1LwzAUhoMoc05_glBEvGvNV5PGC2EWPwYTbyZ4F9I2YZ3dMpMWt39vysruTSAH8j7nJDwA3CCYoLDuVwmiHMecpV8JEiJN2gIiyGmyOwHjY3QKxhAzFmc4pefgwvsVhDBDWIzAiKeBwGIM8pm3jWpru4msiTqnyrqJVLezrbPbpY-Ms-voqXPfS9tU2tUqKvU2QCqaLvI8CqMRuwRnRjVeXw11Aj5fnhf5Wzz_eJ3l03lcUixYTA2tDDak5JxkzBiqmeIVFgXJUooZTVmRZshAzRHHkBmFRThxuElhIUhGJuDuMHfr7E-nfSvXtS9106iNtp2XiEOCBMEBfDiApbPeO23k1tVr5fYSQdkblCvZa5K9JtkblINBuQvN18MrXbHW1bF1UBby2yFXvlSNcWpT1v6IYRE2YgF7PGC_daP3__iAnE_fCWHkD92Hi2k</recordid><startdate>199511</startdate><enddate>199511</enddate><creator>Li, K.</creator><creator>West, T.P.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>199511</creationdate><title>Isolation of uracil auxotrophs from Burkholderia cepacia ATCC 25416</title><author>Li, K. ; West, T.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4296-4f4df2f3c77386ff4e6a7d29b385426456b581f0e717206fa2906f21f050b9383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Aspartate Carbamoyltransferase - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Burkholderia cepacia</topic><topic>Burkholderia cepacia - enzymology</topic><topic>Burkholderia cepacia - metabolism</topic><topic>Cycloserine - metabolism</topic><topic>Dihydroorotase - metabolism</topic><topic>Ethyl Methanesulfonate</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>Mutagenesis</topic><topic>Orotate Phosphoribosyltransferase - metabolism</topic><topic>Orotidine-5'-Phosphate Decarboxylase - metabolism</topic><topic>Pyrimidine Nucleotides - metabolism</topic><topic>Pyrimidines - biosynthesis</topic><topic>Uracil - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, K.</creatorcontrib><creatorcontrib>West, T.P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, K.</au><au>West, T.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of uracil auxotrophs from Burkholderia cepacia ATCC 25416</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>1995-11</date><risdate>1995</risdate><volume>21</volume><issue>5</issue><spage>336</spage><epage>339</epage><pages>336-339</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><coden>LAMIE7</coden><abstract>K. LI AND T.P. WEST. 1995. Two uracil auxotrophs of the phytopathogen Burkholderia cepacia ATCC 25416, which is known to be involved in food spoilage, were isolated by a combination of ethylmethane sulphonate and D‐cycloserine counterselection. One mutant exhibited depressed orotate phosphoribosyltransferase activity while the other mutant lacked orotidine 5'‐monophosphate decarboxylase activity. Pyrimidine limitation of either auxotroph elevated aspartate transcarbamoylase and dihydroorotase activities by at least 1.5‐fold indicating that these pathway enzymes may be repressible by a uracil‐related compound in B. cepacia. Overall, regulation of de novo pyrimidine synthesis in the uracil auxotrophs of B. cepacia ATCC 25416 was observed.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7576529</pmid><doi>10.1111/j.1472-765X.1995.tb01074.x</doi><tpages>4</tpages></addata></record>
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source	Alma/SFX Local Collection
subjects	Aspartate Carbamoyltransferase - metabolism Bacteriology Biological and medical sciences Burkholderia cepacia Burkholderia cepacia - enzymology Burkholderia cepacia - metabolism Cycloserine - metabolism Dihydroorotase - metabolism Ethyl Methanesulfonate Fundamental and applied biological sciences. Psychology Metabolism. Enzymes Microbiology Mutagenesis Orotate Phosphoribosyltransferase - metabolism Orotidine-5'-Phosphate Decarboxylase - metabolism Pyrimidine Nucleotides - metabolism Pyrimidines - biosynthesis Uracil - metabolism
title	Isolation of uracil auxotrophs from Burkholderia cepacia ATCC 25416
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