Elevated Serum MicroRNA Let-7c in Moyamoya Disease

• Published in: Journal of stroke and cerebrovascular diseases 2015-08, Vol.24 (8), p.1709-1714
• Main Authors: Zhao, Shaoyun, MD, Gong, Zhe, MD, Zhang, Jing, MD, Xu, Xiaoge, MD, Liu, Peidong, MD, Guan, Wenjuan, MD, PhD, Jing, Lijun, MD, PhD, Peng, Tao, MD, PhD, Teng, Junfang, MD, Jia, Yanjie, MD, PhD
• Format: Article
• Language: English
• Subjects: Adult; Autoimmune Diseases - blood; Cardiovascular; Cerebral Hemorrhage - blood; Cerebral Infarction - blood; Female; HEK293 Cells; Humans; let-7c; Male; MicroRNAs - blood; MicroRNAs - genetics; Middle Aged; Moyamoya disease; Moyamoya Disease - blood; Neurology; PCR; RNA, Messenger - metabolism; RNF213; Statistics, Nonparametric; Transfection
• ISSN: 1052-3057; 1532-8511
• DOI: 10.1016/j.jstrokecerebrovasdis.2015.01.041

Background Few studies have examined the relationship between mircroRNAs and moyamoya disease (MMD). We performed a study of the significance of let-7c expression in the serum of MMD patients. Methods The experimental group includes 49 MMD patients, and the control group consists of 30 normal people, 20 cerebral hemorrhage patients, 20 massive cerebral infarction patients, 20 nonmassive cerebral infarction patients, and 20 neurological autoimmune disease patients. Let-7 family levels were determined by polymerase chain reaction. A dual luciferase assay was used to test whether let-7c recognized the 3′UTR of RNF213. Results The expression level of let-7c in MMD patients is higher than that observed in the control groups ( P