Analysis of Wnt/Engrailed signaling in Xenopus embryos using biolistics

We adapted the Biolistics Particle Delivery System for the introduction of DNA into Xenopus embryos, allowing us to modulate the expression of different genes at specific time points during development. In the present study we applied the Biolistics method to the study of the wnt-engrailed signaling...

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Bibliographic Details
Published in:Developmental biology 1996-01, Vol.171 (1), p.348-352
Main Authors: Koster, J G, Eizema, K, Peterson-Maduro, L J, Stegeman, B I, Destree, OHJ
Format: Article
Language:English
Subjects:
Freshwater
Xenopus laevis
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Summary:We adapted the Biolistics Particle Delivery System for the introduction of DNA into Xenopus embryos, allowing us to modulate the expression of different genes at specific time points during development. In the present study we applied the Biolistics method to the study of the wnt-engrailed signaling cascade in the developing Xenopus embryo. We show that ectopic expression of Xwnt-1 and Xwnt-5C is sufficient to activate specifically XEn-1 and not XEn-2.
ISSN:0012-1606