Sperm DNA quality evaluated by comet assay and sperm chromatin structure assay in stallions after unilateral orchiectomy

Unilateral orchiectomy (UO) may interfere with thermoregulation of the remaining testis caused by inflammation surrounding the incision site, thus altering normal spermatogenesis and consequently sperm quality. Two measures of sperm DNA quality (neutral comet assay and the sperm chromatin structure...

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Bibliographic Details
Published in:Theriogenology 2015-09, Vol.84 (5), p.833-837
Main Authors: Serafini, R., Varner, D.D., Bissett, W., Blanchard, T.L., Teague, S.R., Love, C.C.
Format: Article
Language:English
Subjects:
Animals
Chromatin - ultrastructure
Comet
Comet Assay - veterinary
DNA
DNA Damage
Horses
Male
Orchiectomy - adverse effects
Orchiectomy - veterinary
Semen Analysis - methods
Semen Analysis - veterinary
Sperm
Stallion
Testis
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Summary:Unilateral orchiectomy (UO) may interfere with thermoregulation of the remaining testis caused by inflammation surrounding the incision site, thus altering normal spermatogenesis and consequently sperm quality. Two measures of sperm DNA quality (neutral comet assay and the sperm chromatin structure assay [SCSA]) were compared before UO (0 days) and at 14, 30, and 60 days after UO to determine whether sperm DNA changed after a mild testis stress (i.e., UO). The percent DNA in the comet tail was higher at 14 and 60 days compared to 0 days (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2015.05.024