Anti-tumor effect of LTA combined with 5-FU on H22 tumor bearing mice

• Published in: Asian Pacific journal of tropical medicine 2015-07, Vol.8 (7), p.560-564
• Main Authors: Wang, Bin, Lei, Chang-Jiang, Wu, Rong, Li, Lei, Deng, Chun-Mei, Chen, Wen-Xia, Hu, Fu-Rong, Long, Hao-Cheng, Tao, Ze-Zhang, Zeng, Cheng, Huang, Jian-Bin, Chen, Chun-Zhou, Ren, De-Fa
• Format: Article
• Language: English
• Subjects: 5-FU; Caspase-3; EGFR; LTA; TGF-α; Tumor inhibition rate
• ISSN: 1995-7645; 2352-4146
• DOI: 10.1016/j.apjtm.2015.07.002

To study the effect of lipoteichoic acid (LTA) and 5-FU on the expression of caspase-3, EGFR, TGF-α proteins of tumor tissue of H22 cancer bearing mice and its anti-tumor mechanism. A total of 40 SPF grade Kunming mice were selected to establish H22 liver cancer model, and then the mice were divided into 4 groups at random with ten mice in each group. Group A was given saline lavage treatment, Group B was treated with 5-FU by intraperitoneal injection, Group C was treated with LTA by lump body injection; Group D was treated with LTA by lump body injection and 5-FU by intraperitoneal injection. Two weeks after the treatment, the mice in each group were executed and the tumor tissue was stripping and weighted, and the tumor growth inhibition ratio was calculated. Then the tumor tissue was processed for conventional embedding, sectioned to observe the expression of caspase-3, EGFR, TGF-α by immunohistochemical staining method. The tumor inhibitory rate o f Group D was significantly higher than Groups B and C (P  0.05). The IDO values of TGF-α, EGFR proteins in Groups B, C, D mice tumor tissue were significantly lower than that in group A (P