A precise spectrophotometric method for measuring sodium dodecyl sulfate concentration

Sodium dodecyl sulfate (SDS) is used to denature and solubilize proteins, especially membrane and other hydrophobic proteins. A quantitative method to determine the concentration of SDS using the dye Stains-All is known. However, this method lacks the accuracy and reproducibility necessary for use w...

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Bibliographic Details
Published in:Analytical biochemistry 2015-10, Vol.486, p.78-80
Main Authors: Rupprecht, Kevin R., Lang, Ewa Z., Gregory, Svetoslava D., Bergsma, Janet M., Rae, Tracey D., Fishpaugh, Jeffrey R.
Format: Article
Language:English
Subjects:
Concentration
Hydrogen-Ion Concentration
Light
Limit of Detection
Quantitative analysis
Reproducibility of Results
Sodium dodecyl sulfate (SDS)
Sodium Dodecyl Sulfate - analysis
Solvents - chemistry
Spectrophotometry - methods
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Summary:Sodium dodecyl sulfate (SDS) is used to denature and solubilize proteins, especially membrane and other hydrophobic proteins. A quantitative method to determine the concentration of SDS using the dye Stains-All is known. However, this method lacks the accuracy and reproducibility necessary for use with protein solutions where SDS concentration is a critical factor, so we modified this method after examining multiple parameters (solvent, pH, buffers, and light exposure). The improved method is simple to implement, robust, accurate, and (most important) precise.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2015.06.013